| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPK14 |
| Uniprot No | Q16539 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-360aa |
| 氨基酸序列 | SQERPTFYRQELNKTIWEVPERYQNLSPVGSGAYGSVCAAFDTKTGLRVAVKKLSRPFQSIIHAKRTYRELRLLKHMKHENVIGLLDVFTPARSLEEFNDVYLVTHLMGADLNNIVKCQKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILDFGLARHTDDEMTGYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTGRTLFPGTDHIDQLKLILRLVGTPGAELLKKISSESARNYIQSLTQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAYFAQYHDPDDEPVADPYDQSFESRDLLIDEWKSLTYDEVISFVPPPLDQEEMES |
| 预测分子量 | 72.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
nan
MAPK14 (mitogen-activated protein kinase 14), also known as p38α, is a serine/threonine kinase belonging to the evolutionarily conserved MAPK family. It plays a central role in cellular responses to stress signals (e.g., cytokines, UV radiation, osmotic shock) and regulates processes like inflammation, apoptosis, cell differentiation, and proliferation. The human MAPK14 gene encodes a 360-amino acid protein with a conserved kinase domain and phosphorylation motifs (Thr180-Gly-Tyr182) critical for activation.
Recombinant MAPK14 protein is typically produced using expression systems like *E. coli*, insect cells, or mammalian cells (e.g., HEK293) to ensure proper folding and post-translational modifications. Purification often involves affinity tags (e.g., GST, His-tag) for downstream applications. Its recombinant form enables mechanistic studies of p38α signaling pathways, including interactions with upstream kinases (MKK3/6) and downstream substrates (e.g., MAPKAPK2. transcription factors).
As a therapeutic target, MAPK14 is implicated in inflammatory diseases (e.g., rheumatoid arthritis), neurodegenerative disorders, and cancer. Recombinant MAPK14 facilitates drug screening, inhibitor characterization, and structural studies (e.g., X-ray crystallography) to design selective kinase inhibitors. However, challenges remain in achieving isoform specificity due to high homology within the p38 subfamily (p38β, γ, δ). Current research leverages recombinant protein tools to dissect its dual roles in pro-inflammatory and tumor-suppressive contexts, aiming to develop context-dependent therapies. Its utility extends to biomarker discovery and pathway validation in preclinical models, underscoring its importance in biomedical research.
×
