| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPK11 |
| Uniprot No | Q15759 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-364aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMSGPRAGFYRQELNKTVWEVPQRLQGL RPVGSGAYGSVCSAYDARLRQKVAVKKLSRPFQSLIHARRTYRELRLLKH LKHENVIGLLDVFTPATSIEDFSEVYLVTTLMGADLNNIVKCQALSDEHV QFLVYQLLRGLKYIHSAGIIHRDLKPSNVAVNEDCELRILDFGLARQADE EMTGYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLQGKALFPGSD YIDQLKRIMEVVGTPSPEVLAKISSEHARTYIQSLPPMPQKDLSSIFRGA NPLAIDLLGRMLVLDSDQRVSAAEALAHAYFSQYHDPEDEPEAEPYDESV EAKERTLEEWKELTYQEVLSFKPPEPPKPPGSLEIEQ |
| 预测分子量 | 44 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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**Background of MAPK11 Recombinant Protein**
Mitogen-activated protein kinase 11 (MAPK11), also known as p38β, is a member of the serine/threonine-specific p38 MAPK family, which plays pivotal roles in cellular responses to stress, inflammation, apoptosis, and differentiation. It is activated by upstream kinases (MKK3/6) through phosphorylation of conserved threonine and tyrosine residues, triggering downstream signaling cascades. MAPK11 shares structural and functional similarities with other p38 isoforms (e.g., p38α) but exhibits distinct tissue distribution and substrate preferences, influencing its biological specificity.
Recombinant MAPK11 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce high-purity, functional kinase for research. This involves cloning the MAPK11 gene into expression vectors, followed by purification via affinity tags (e.g., His-tag). The recombinant protein retains enzymatic activity, enabling *in vitro* studies on substrate interactions, signaling mechanisms, and inhibitor screening.
MAPK11 is implicated in diseases such as cancer, autoimmune disorders, and neurodegeneration, making it a therapeutic target. Researchers utilize recombinant MAPK11 to investigate its role in pathways like NF-κB and STAT3. as well as its crosstalk with other kinases. Additionally, it aids in developing selective inhibitors to minimize off-target effects observed with pan-p38 inhibitors.
In summary, MAPK11 recombinant protein serves as a critical tool for dissecting p38 MAPK signaling, advancing drug discovery, and understanding its physiological and pathological contributions.
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