| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPK1 |
| Uniprot No | P28482 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-360aa |
| 氨基酸序列 | AAAAAAGAG PEMVRGQVFD VGPRYTNLSY IGEGAYGMVC SAYDNVNKVR VAIKKISPFE HQTYCQRTLR EIKILLRFRH ENIIGINDII RAPTIEQMKD VYIVQDLMET DLYKLLKTQH LSNDHICYFL YQILRGLKYI HSANVLHRDL KPSNLLLNTT CDLKICDFGL ARVADPDHDH TGFLTEYVAT RWYRAPEIML NSKGYTKSID IWSVGCILAE MLSNRPIFPG KHYLDQLNHI LGILGSPSQE DLNCIINLKA RNYLLSLPHK NKVPWNRLFP NADSKALDLL DKMLTFNPHK RIEVEQALAH PYLEQYYDPS DEPIAEAPFK FDMELDDLPK EKLKELIFEE TARFQPGYRS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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**Background of MAPK1 Recombinant Protein**
Mitogen-activated protein kinase 1 (MAPK1), also known as extracellular signal-regulated kinase 2 (ERK2), is a conserved serine/threonine kinase central to the MAPK signaling cascade. It plays a pivotal role in transmitting extracellular signals—such as growth factors, cytokines, and stress stimuli—to regulate cellular responses, including proliferation, differentiation, survival, and apoptosis. MAPK1 is activated through dual phosphorylation by upstream MAPK kinases (MEK1/2) at threonine and tyrosine residues within its activation loop, triggering its translocation to the nucleus to modulate gene expression via transcription factors like c-Fos and c-Jun.
Recombinant MAPK1 protein is engineered using expression systems (e.g., *E. coli*, insect, or mammalian cells*) to produce a purified, bioactive form of the enzyme for *in vitro* studies. This engineered protein retains functional domains critical for kinase activity, substrate binding, and regulatory interactions. Its production often involves affinity chromatography tags (e.g., GST, His-tag) to facilitate purification and detection. Researchers employ recombinant MAPK1 to dissect signaling mechanisms, screen kinase inhibitors, or study dysregulated pathways in diseases such as cancer, neurodegenerative disorders, and inflammation.
The use of recombinant MAPK1 overcomes challenges associated with isolating the native protein from tissues, ensuring consistent quality, high purity, and activity. Importantly, phosphorylation at specific residues (T185/Y187) is often validated to confirm functionality. Studies leveraging recombinant MAPK1 have advanced drug discovery, particularly in targeting hyperactive ERK signaling in RAS/RAF-mutant cancers. Additionally, it serves as a tool to explore crosstalk with other pathways, including PI3K/AKT and JNK, highlighting its integrative role in cellular decision-making.
In summary, recombinant MAPK1 is a vital resource for unraveling kinase-driven signaling networks and developing targeted therapies.
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