| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAOA |
| Uniprot No | P21397 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-497aa |
| 氨基酸序列 | MHHHHHHDYKDDDDKENQEKASIAGHMFDVVVIGGGISGLSAAKLLTEYG VSVLVLEARDRVGGRTYTIRNEHVDYVDVGGAYVGPTQNRILRLSKELGI ETYKVNVSERLVQYVKGKTYPFRGAFPPVWNPIAYLDYNNLWRTIDNMGK EIPTDAPWEAQHADKWDKMTMKELIDKICWTKTARRFAYLFVNINVTSEP HEVSALWFLWYVKQCGGTTRIFSVTNGGQERKFVGGSGQVSERIMDLLGD QVKLNHPVTHVDQSSDNIIIETLNHEHYECKYVINAIPPTLTAKIHFRPE LPAERNQLIQRLPMGAVIKCMMYYKEAFWKKKDYCGCMIIEDEDAPISIT LDDTKPDGSLPAIMGFILARKADRLAKLHKEIRKKKICELYAKVLGSQEA LHPVHYEEKNWCEEQYSGGCYTAYFPPGIMTQYGRVIRQPVGRIFFAGTE TATKWSGYMEGAVEAGERAAREVLNGLGKVTEKDIWVQEPESKDVPAVEI THTFWERNLPS |
| 预测分子量 | 58 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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The monoamine oxidase A (MAOA) enzyme, encoded by the *MAOA* gene on the X chromosome, plays a critical role in neurotransmitter homeostasis by catalyzing the oxidative deamination of monoamine neurotransmitters, including serotonin, norepinephrine, and dopamine. This process regulates neurotransmitter levels in the brain and peripheral tissues, impacting mood, behavior, and neurological function. Dysregulation of MAOA activity has been linked to neuropsychiatric disorders such as depression, anxiety, and aggression, as well as neurodegenerative conditions like Parkinson’s disease.
Recombinant MAOA protein, produced via genetic engineering in heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells), enables detailed biochemical and pharmacological studies. Its production involves cloning the *MAOA* gene into expression vectors, followed by purification using affinity chromatography. Recombinant MAOA retains catalytic activity, allowing researchers to investigate substrate specificity, inhibitor interactions, and structural-functional relationships.
This protein is particularly valuable in drug discovery, as MAOA inhibitors are therapeutic targets for depression and neurodegenerative diseases. Recombinant MAOA facilitates high-throughput screening of potential inhibitors and mechanistic studies of enzyme kinetics. Additionally, it aids in exploring genetic variants (e.g., the "warrior gene" polymorphism) that alter enzyme activity and disease susceptibility.
Beyond neuroscience, recombinant MAOA is used to study its role in peripheral tissues, such as modulating catecholamine metabolism in the cardiovascular system. Its application extends to developing biosensors and diagnostic tools for monitoring neurotransmitter dynamics. Overall, recombinant MAOA serves as a vital tool for unraveling the enzyme's biological significance and advancing targeted therapies for MAOA-associated disorders.
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