首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人凝聚素(CLU)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、唾液、尿液样本中的CLU含量。人凝聚素（CLU）是一种分泌蛋白，主要在大脑的星形胶质细胞中合成，与阿尔茨海默病（AD）的发病机制密切相关。研究显示，CLU通过调控淀粉样蛋白的形成，影响AD的发生和发展，其作用机制涉及促进Aβ生成和神经斑块的形成。试剂盒检测范围为3.12 ng/mL-200 ng/mL，灵敏度为0.78 ng/mL。该产品适用于基础医学研究领域，例如通过检测不同疾病模型或药物处理后的样本中CLU表达水平，探究其在疾病发生发展中的作用机制，或作为生物标志物用于分子通路研究、药物疗效评估及细胞功能实验的辅助分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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缩写：CLU
Uniprot No.：P10909
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, saliva, urine
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CLU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:2000 | Average % | 97 | ||||
| Range % | 91-104 | |||||
| 1:4000 | Average % | 92 | ||||
| Range % | 85-100 | |||||
| 1:8000 | Average % | 93 | ||||
| Range % | 83-99 | |||||
回收率：
| The recovery of human CLU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 92-106 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 2002.2482.2052.2272.072 1001.1961.2661.2311.076 500.6750.6630.6690.514 250.4720.4530.4630.308 12.50.3350.3140.3250.170 6.250.2210.2070.2140.059 3.120.2000.1940.1970.042 00.1550.1540.155 | ||||||
货期：3-5 working days
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