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Human Tetranectin(CLEC3B) ELISA kit

  • 中文名: 人四连接素(CLEC3B) ELISA kit
货号: IPD-EL005531HU
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人四连接素(CLEC3B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的CLEC3B含量。人四连接素(CLEC3B)是一种钙离子依赖性凝集素,参与细胞黏附、分化调控及肿瘤抑制。其与纤溶酶原和kringle 4结合,可能涉及胞吐作用相关分子包装和眼部生理学。研究显示,CLEC3B在多种癌症中表达下调,与肿瘤侵袭、转移和免疫微环境相关,具有潜在的诊断和预后价值。试剂盒检测范围为1.56 ng/ml- 100 ng/ml,灵敏度为0.39 ng/ml。支持科研人员在体外实验中高效检测目标蛋白的表达变化,适用于基础医学研究中疾病机制探索、生物标志物筛选以及药物干预效果评估等场景。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    C type lectin domain family 3 member B ELISA Kit; C-type lectin domain family 3 member B ELISA Kit; Clec3b ELISA Kit; Plasminogen kringle 4 binding protein ELISA Kit; Plasminogen kringle 4-binding protein ELISA Kit; TETN_HUMAN ELISA Kit; Tetranectin (plasminogen binding protein) ELISA Kit; Tetranectin ELISA Kit; TN ELISA Kit; TNA ELISA Kit
  • 缩写:
    CLEC3B
  • Uniprot No.:
    P05452
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    1.56 ng/ml- 100 ng/ml
  • 灵敏度:
    0.39 ng/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human CLEC3B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:100

    Average %

    107


    Range %

    103-112


    1:200

    Average %

    101


    Range %

    94-107


    1:400

    Average %

    95


    Range %

    88-101


    1:800

    Average %

    99


    Range %

    94-105


  • 回收率:

    The recovery of human CLEC3B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-100


    EDTA plasma (n=4)

    99

    92-106


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    100

    2.861

    2.734

    2.798

    2.655


    50

    1.897

    1.867

    1.882

    1.739


    25

    1.111

    1.058

    1.084

    0.942


    12.5

    0.649

    0.629

    0.639

    0.496


    6.25

    0.420

    0.399

    0.410

    0.267


    3.12

    0.290

    0.283

    0.287

    0.144


    1.56

    0.209

    0.204

    0.206

    0.064


    0

    0.141

    0.145

    0.143




  • 货期:
    3-5 working days

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