关键信息
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基因名
ENPP-2
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简介
The ENPP-2 protein catalyzes the hydrolysis of lysophospholipids (especially lysophosphatidylcholine) to produce the signaling molecule lysophosphatidic acid (LPA).It also acts on sphingosine phosphocholine to produce sphingosine 1-phosphate, which affects cell movement.ENPP-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ENPP-2 protein, expressed by HEK293 , with N-10*His labeled tag.
- 应用
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别名
Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; Autotaxin; LysoPLD; Npps2; Pdnp2
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种属
Mouse
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表达系统
HEK293
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标签
N-10*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q9R1E6-1
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表达区间
S49-I862
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氨基酸序列
SPWTNTSGSCKGRCFELQEVGPPDCRCDNLCKSYSSCCHDFDELCLKTARGWECTKDRCGEVRNEENACHCSEDCLSRGDCCTNYQVVCKGESHWVDDDCEEIRVPECPAGFVRPPLIIFSVDGFRASYMKKGSKVMPNIEKLRSCGTHAPYMRPVYPTKTFPNLYTLATGLYPESHGIVGNSMYDPVFDATFHLRGREKFNHRWWGGQPLWITATKQGVRAGTFFWSVSIPHERRILTILQWLSLPDNERPSVYAFYSEQPDFSGHKYGPFGPEMTNPLREIDKTVGQLMDGLKQLKLHRCVNVIFVGDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTLGRIRPKIPNNLKYDPKAIIANLTCKKPDQHFKPYMKQHLPKRLHYANNRRIEDLHLLVERRWHVARKPLDVYKKPSGKCFFQGDHGFDNKVNSMQTVFVGYGPTFKYRTKVPPFENIELYNVMCDLLGLKPAPNNGTHGSLNHLLRTNTFRPTLPEEVSRPNYPGIMYLQSDFDLGCTCDDKVEPKNKLEELNKRLHTKGSTEERHLLYGRPAVLYRTSYDILYHTDFESGYSEIFLMPLWTSYTISKQAEVSSIPEHLTNCVRPDVRVSPGFSQNCLAYKNDKQMSYGFLFPPYLSSSPEAKYDAFLVTNMVPMYPAFKRVWTYFQRVLVKKYASERNGVNVISGPIFDYNYNGLRDIEDEIKQYVEGSSIPVPTHYYSIITSCLDFTQPADKCDGPLSVSSFILPHRPDNDESCNSSEDESKWVEELMKMHTARVRDIEHLTGLDFYRKTSRSYSEILTLKTYLHTYESEI
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蛋白长度
Partial
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分子量
100 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
ENPP-2, also known as autotaxin, is a key enzyme involved in the hydrolysis of lysophosphatidylcholine (LPC) to produce lysophosphatidic acid (LPA), a bioactive lipid mediator that plays crucial roles in various physiological processes including cell proliferation, migration, and survival. Elevated levels of LPA are associated with several pathological conditions, such as cancer progression, inflammatory diseases, and neurological disorders. The study of ENPP-2 has garnered significant attention due to its potential as a therapeutic target for these illnesses. Recombinant ENPP-2 protein production allows researchers to investigate its structure, function, and regulation in greater detail, facilitating the development of ENPP-2 inhibitors or modulators. Understanding the enzymatic activity and molecular interactions of ENPP-2 can provide insights into its role in cellular signaling pathways and disease mechanisms, ultimately contributing to the identification of novel treatment strategies. Additionally, recombinant ENPP-2 can be utilized in various biochemical assays and drug screening processes, helping to fast-track the discovery of effective therapeutic agents aimed at modulating ENPP-2 activity. As research continues to unravel the complexities of ENPP-2's functions, it holds promise as a pivotal component in the pursuit of advanced therapies for a range of debilitating conditions.
