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Rat Chitinase-3-like protein 1(CHI3L1) ELISA kit

  • 中文名: 大鼠几丁质酶3样蛋白1(CHI3L1)酶联免疫试剂盒
货号: IPD-EL005346RA
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    大鼠几丁质酶3样蛋白1(CHI3L1)酶联免疫试剂盒检测血清、血浆、组织匀浆样本中的CHI3L1含量。CHI3L1是一个重要靶点。它与多种疾病发生发展相关,如炎症、肿瘤等。研究机制上,其可能通过参与细胞增殖、迁移、凋亡等过程,影响疾病进程,目前围绕它开展的研究有助于深入理解疾病发病机理,为相关疾病治疗提供新思路。试剂盒检测范围为4.7 ng/ml- 300 ng/ml,灵敏度为1.17 ng/ml。研究呼吸系统疾病、肝纤维化及肿瘤微环境等病理机制;炎症相关疾病模型构建、纤维化进程评估及药物干预效果研究;探索CHI3L1在生理病理过程中的分子机制本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Chi3l1Chitinase-3-like protein 1 ELISA kit; Cartilage glycoprotein 39 ELISA kit; CGP-39 ELISA kit; GP-39 ELISA kit
  • 缩写:
    CHI3L1
  • Uniprot No.:
    Q9WTV1
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    4.7 ng/ml - 300 ng/ml
  • 灵敏度:
    1.17 ng/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of rat CHI3L1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    103


    Range %

    99-108


    1:2

    Average %

    98


    Range %

    94-102


    1:4

    Average %

    93


    Range %

    89-97


    1:8

    Average %

    90


    Range %

    86-98


  • 回收率:

    The recovery of rat CHI3L1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    90-100


    EDTA plasma (n=4)

    89

    84-95


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    300

    2.486

    2.358

    2.422

    2.241


    150

    2.043

    1.931

    1.987

    1.806


    75

    1.241

    1.329

    1.285

    1.104


    37.5

    0.765

    0.803

    0.784

    0.603


    18.75

    0.413

    0.427

    0.420

    0.239


    9.4

    0.339

    0.345

    0.342

    0.161


    4.7

    0.231

    0.224

    0.228

    0.047


    0

    0.180

    0.182

    0.181




  • 货期:
    3-5 working days

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