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产品描述：人几丁质酶3样蛋白1(YKL-40/CHI3L1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CHI3L1含量。人几丁质酶3样蛋白1（YKL-40/CHI3L1）是一种炎症分子，主要在脑星形胶质细胞中表达，与神经炎症和阿尔茨海默病等神经退行性疾病有关。其通过调节免疫细胞活性、促进细胞增殖和存活，以及影响组织重塑和血管生成等途径，在神经炎症和疾病发展中发挥重要作用。试剂盒检测范围为46.875 pg/mL-3000 pg/mL，灵敏度为78.065 pg/mL。科研人员可通过探究YKL-40在心血管疾病、呼吸系统疾病或肿瘤微环境中的调控机制，或用于评估实验模型中炎症相关生物标志物的动态变化，为疾病机制研究及药物干预效果分析提供技术支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：39 kDa synovial protein ELISA Kit; ASRT7 ELISA Kit; Cartilage glycoprotein 39 ELISA Kit; CGP-39 ELISA Kit; CGP39 ELISA Kit; CH3L1_HUMAN ELISA Kit; CHI3L1 ELISA Kit; chitinase 3 like 1 (cartilage glycoprotein 39) ELISA Kit; chitinase 3 like 1 ELISA Kit; Chitinase 3 like protein 1 precursor ELISA Kit; chitinase ELISA Kit; Chitinase-3-like protein 1 ELISA Kit; Chondrocyte protein YKL40 ELISA Kit; GP 39 ELISA Kit; GP-39 ELISA Kit; GP39 ELISA Kit; HC gp39 ELISA Kit; HCGP 3P ELISA Kit; hCGP-39 ELISA Kit; HCgp39 ELISA Kit; YKL 40 ELISA Kit; YKL-40 ELISA Kit; YKL40 ELISA Kit; YYL 40 ELISA Kit
缩写：CHI3L1
Uniprot No.：P36222
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：46.875 pg/mL-3000 pg/mL
灵敏度：78.065 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human Chitinase-3-like Protein 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human CHI3L1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled CHI3L1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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