Human Adipsin ELISA Kit

货号: IPD-E14369h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人降脂素酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CFD含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，灵敏度为0.078 ng/mL。适用于基础医学研究中脂代谢调控机制探索、心血管疾病模型分析、以及降脂药物或营养干预的体外效果评估；支持科研人员高效完成细胞实验、动物模型或临床前样本的定量分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Adipsin ELISA Kit; Adipsin/complement factor D ELISA Kit; Adn ELISA Kit; C3 convertase activator ELISA Kit; CFAD_HUMAN ELISA Kit; CFD ELISA Kit; Complement factor D ELISA Kit; complement factor D preproprotein ELISA Kit; D component of complement ELISA Kit; DF ELISA Kit; FactorD ELISA Kit; PFD ELISA Kit; Properdin factor D ELISA Kit
缩写：
CFD
Uniprot No.：
P00746
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.312 ng/mL-20 ng/mL
灵敏度：
0.078 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)2.5932.5592.6192.6712.6402.602SD0.0430.0360.0390.0570.0500.048CV(%)5.0894.3014.5836.6055.8415.667

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human adipsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:500	Average %	87
	Range %	82-91
1:1000	Average %	84
	Range %	80-88
1:2000	Average %	104
	Range %	98-108
1:4000	Average %	90
	Range %	85-94

回收率：

The recovery of human adipsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-103
EDTA plasma (n=4)	94	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
20	2.591	2.423	2.507	2.402
10	1.964	1.890	1.927	1.822
5	1.336	1.301	1.319	1.214
2.5	0.842	0.836	0.839	0.734
1.25	0.449	0.424	0.437	0.332
0.63	0.329	0.339	0.334	0.229
0.31	0.249	0.241	0.245	0.140
0	0.108	0.102	0.105