| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAFK |
| Uniprot No | O60675 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-156aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MTTNPKPNKA LKVKKEAGEN APVLSDDELV SMSVRELNQH LRGLTKEEVT RLKQRRRTLK NRGYAASCRI KRVTQKEELE RQRVELQQEV EKLARENSSM RLELDALRSK YEALQTFART VARGPVAPSK VATTSVITIV KSTELSSTSV PFSAAS |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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MAFK (Musculoaponeurotic Fibrosarcoma Oncogene Homolog K) is a member of the Maf family of basic leucine zipper (bZIP) transcription factors, which regulate gene expression by binding to DNA response elements. Discovered in the 1990s, MAFK plays critical roles in cellular stress response, differentiation, and homeostasis. It forms heterodimers with other bZIP proteins, particularly CNC (Cap'n'Collar) family members like NRF1 and NRF2. to activate antioxidant response element (ARE)-driven genes. This interaction is central to the oxidative stress defense mechanism, enabling cells to neutralize reactive oxygen species (ROS) and maintain redox balance.
Recombinant MAFK protein is engineered for in vitro studies to dissect its structural and functional properties. Produced using expression systems like Escherichia coli or mammalian cell cultures, the recombinant protein retains DNA-binding activity and dimerization capabilities. Researchers utilize it to study MAFK's role in signaling pathways, its interplay with binding partners (e.g., NRF2 in cytoprotective responses), and its involvement in diseases such as cancer, neurodegenerative disorders, and chronic inflammation. For instance, MAFK dysregulation has been linked to tumor progression and chemoresistance, making it a potential therapeutic target.
The development of recombinant MAFK also aids in high-throughput screening for drug candidates targeting MAFK-dependent pathways. Its purified form enables crystallography and biochemical assays, advancing structural insights into bZIP domain interactions. As a tool, recombinant MAFK bridges molecular biology and translational research, offering avenues to explore cellular adaptation mechanisms and design precision therapies against oxidative stress-related pathologies.
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