Human Soluble Cluster of differentiation 40 ligand,sCD40L ELISA Kit

中文名： 人可溶性CD40配体(sCD40L)ELISA Kit

货号: IPD-E04716h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人可溶性CD40配体(sCD40L)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD40LG含量。CD40LG是重要靶点。它参与免疫调节，与CD40结合激活B细胞等免疫细胞。在肿瘤免疫等领域研究较多，其阻断或激活机制探究，有望开发新的免疫治疗策略，为癌症等疾病带来新的治疗思路和方法。试剂盒检测范围为0.312 ng/mL-20 ng/mL，灵敏度为0.078 ng/mL。适用于基础医学研究中sCD40L在免疫调控、疾病发生机制中的功能探索，或作为炎症相关药物开发的体外检测工具，为评估生物标志物表达水平提供可靠技术支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
CD 40L ELISA Kit; CD154 ELISA Kit; CD40 antigen ligand ELISA Kit; CD40 ligand ELISA Kit; CD40 ligand; soluble form ELISA Kit; CD40-L ELISA Kit; CD40L ELISA Kit; CD40L_HUMAN ELISA Kit; CD40LG ELISA Kit; gp39 ELISA Kit; hCD40L ELISA Kit; HIGM1 ELISA Kit; IGM ELISA Kit; IMD3 ELISA Kit; T B cell activating molecule ELISA Kit; T BAM ELISA Kit; T-cell antigen Gp39 ELISA Kit; TNF-related activation protein ELISA Kit; TNFSF5 ELISA Kit; TrAP ELISA Kit; Tumor necrosis factor (ligand) superfamily member 5 ELISA Kit; Tumor necrosis factor ligand superfamily member 5 ELISA Kit
缩写：
CD40LG
Uniprot No.：
P29965
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.312 ng/mL-20 ng/mL
灵敏度：
0.078 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)2.5002.4892.5102.4762.5142.507SD0.0340.0290.0410.0540.0580.052CV(%)3.7403.2014.4965.9836.3535.708

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human sCD40L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

85-101

1:2

Average %

Range %

82-94

1:4

Average %

Range %

82-105

1:8

Average %

Range %

82-100

回收率：

The recovery of human sCD40L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-100
Platelet-poor EDTA plasma (n=4)	93	85-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.











ng/ml	OD1	OD2	Average	Corrected
20	2.653	2.756	2.705	2.568
10	2.108	2.215	2.162	2.025
5	1.524	1.505	1.515	1.378
2.5	0.878	0.860	0.869	0.732
1.25	0.575	0.543	0.559	0.422
0.625	0.347	0.334	0.341	0.204
0.312	0.258	0.261	0.260	0.123
0	0.141	0.132	0.137