产品描述：人可溶性CD14(sCD14)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、尿液样本中的CD14含量。CD14是一种细胞表面蛋白，是天然免疫中重要的模式识别受体。它可识别细菌脂多糖等病原体相关分子模式，在免疫应答启动中起关键作用。研究围绕其识别机制、信号传导途径展开，以深入理解免疫调控并开发相关治疗策略。试剂盒检测范围为0.234 ng/mL-15 ng/mL，灵敏度为0.229 ng/mL。支持科研人员探索sCD14在脓毒症、慢性炎症疾病或免疫代谢紊乱等研究模型中的动态变化；适用于比较不同样本来源（如循环系统、泌尿系统或体外培养体系）中sCD14的表达特征；满足分子免疫学、疾病机制研究及生物标志物筛选等领域的定量检测需求，为炎症相关信号通路研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD 14 ELISA Kit; CD_antigen=CD14 ELISA Kit; CD14 ELISA Kit; CD14 antigen ELISA Kit; CD14 molecule ELISA Kit; CD14_HUMAN ELISA Kit; LPS-R ELISA Kit; Mo2 ELISA Kit; Monocyte differentiation antigen CD14 ELISA Kit; Monocyte differentiation antigen CD14 urinary form ELISA Kit; Monocyte differentiation antigen CD14; membrane-bound form ELISA Kit; Myeloid cell specific leucine rich glycoprotein ELISA Kit; Myeloid cell-specific leucine-rich glycoprotein ELISA Kit
缩写：CD14
Uniprot No.：P08571
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, urine
检测范围：0.234 ng/mL-15 ng/mL
灵敏度：0.229 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human sCD14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human CD14 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled CD14 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
