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产品描述：人嗜酸粒细胞趋化蛋白Eotaxin 2(Eotaxin 2/CCL24)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL24含量。CCL24，也称为嗜酸性粒细胞趋化蛋白2（eotaxin-2）和骨髓祖细胞抑制因子2（MPIF-2），是CC趋化因子家族的一种小细胞因子，位于人类基因组第7号染色体上。CCL24通过与CCR3受体结合，诱导嗜酸性粒细胞趋化性，介导特应性疾病、寄生虫感染和全身性疾病，并促进细胞运输和调节炎症及纤维化活动。研究显示，褪黑激素能抑制CCL24的表达，从而抑制骨肉瘤细胞的转移和侵袭。试剂盒检测范围为78.125 pg/mL-5000 pg/mL，灵敏度为67.660 pg/mL。该产品适用于基础医学研究中探索过敏性哮喘、特应性皮炎等Th2型免疫反应相关疾病的分子机制，也可用于体外药物筛选模型中评估化合物对CCL24表达水平的调控作用，为炎症微环境研究、免疫细胞趋化行为分析等科研领域提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C C motif chemokine 24 ELISA Kit; C-C motif chemokine 24 ELISA Kit; CCL24 ELISA Kit; CCL24_HUMAN ELISA Kit; Chemokine CC Motif Ligand 24 ELISA Kit; CK beta 6 ELISA Kit; CK-beta-6 ELISA Kit; Ckb6 ELISA Kit; Eosinophil chemotactic protein 2 ELISA Kit; Eotaxin-2 ELISA Kit; MPIF 2 ELISA Kit; MPIF-2 ELISA Kit; MPIF2 ELISA Kit; Myeloid progenitor inhibitory factor 2 ELISA Kit; SCYA24 ELISA Kit; Small inducible cytokine A24 ELISA Kit; Small inducible cytokine subfamily A (Cys-Cys) member 24 ELISA Kit; Small-inducible cytokine A24 ELISA Kit
缩写：CCL24
Uniprot No.：O00175
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：78.125 pg/mL-5000 pg/mL
灵敏度：67.660 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human eotaxin 2/CCL24 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 85-94 | ||||||
| 1:4 | Average % | 96 | |||||
| Range % | 91-101 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 100-108 | ||||||
回收率：
| The recovery of human eotaxin 2/CCL24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 99 | 95-103 | |||||
| EDTA plasma (n=4) | 86 | 83-89 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 50002.1522.2042.1782.065 25001.7961.7351.7661.653 12501.1821.2051.1941.081 6250.7650.7980.7820.669 312.50.4530.4680.4610.348 156.250.3280.3310.3300.217 78.1250.2510.2400.2460.133 00.1150.1100.113 | |||||||
货期：3-5 working days
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