| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAD2L1BP |
| Uniprot No | Q15013 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-274aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMAAPEA EVLSSAAVPD LEWYEKSEET HASQIELLET SSTQEPLNAS EAFCPRDCMV PVVFPGPVSQ EGCCQFTCEL LKHIMYQRQQ LPLPYEQLKH FYRKPSPQAE EMLKKKPRAT TEVSSRKCQQ ALAELESVLS HLEDFFARTL VPRVLILLGG NALSPKEFYE LDLSLLAPYS VDQSLSTAAC LRRLFRAIFM ADAFSELQAP PLMGTVVMAQ GHRNCGEDWF RPKLNYRVPS RGHKLTVTLS CGRPSIRTTA WEDYIWFQAP VTFKGFRE |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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MAD2L1BP (MAD2L1 Binding Protein), also known as p31comet, is a conserved eukaryotic protein that plays a critical role in regulating the spindle assembly checkpoint (SAC), a surveillance mechanism ensuring accurate chromosome segregation during mitosis. It directly interacts with MAD2 (Mitotic Arrest Deficient 2), a core SAC component, to modulate checkpoint signaling. During mitosis, SAC activation delays anaphase until all chromosomes achieve proper microtubule attachment. MAD2L1BP acts as a negative regulator by disrupting the MAD2-CDC20 complex, thereby silencing SAC and promoting cell cycle progression. Dysregulation of this process is linked to chromosomal instability, a hallmark of cancer.
Recombinant MAD2L1BP refers to the protein produced through genetic engineering, typically expressed in bacterial (e.g., *E. coli*) or mammalian systems. Its production enables detailed biochemical and structural studies, including interactions with MAD2. conformational changes, and SAC termination mechanisms. The recombinant protein often includes affinity tags (e.g., His-tag) for purification and detection. Researchers utilize it to investigate checkpoint control, mitotic errors, and cancer-related pathways. Notably, MAD2L1BP overexpression has been observed in certain tumors, suggesting its potential as a therapeutic target. Studies also explore its role in chemoresistance and as a biomarker. Current research focuses on elucidating its structure-function relationships and developing small-molecule inhibitors to disrupt its interaction with MAD2 in cancer cells. Its recombinant form is essential for high-throughput screening assays and mechanistic studies aiming to understand aneuploidy-related diseases.
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