首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠巨噬细胞来源的趋化因子(MDC/CCL22)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液、细胞裂解物样本中的CCL22含量。CCL22是一种趋化因子。它在免疫调节等生理过程中发挥重要作用。在研究机制上，其主要通过与特定受体结合，引导免疫细胞迁移和激活。目前围绕CCL22在肿瘤免疫、自身免疫病等领域有诸多研究，探索其在疾病发生发展中的作用。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，灵敏度为23.672 pg/mL。本产品可广泛应用于免疫学、炎症机制研究及药物开发等科研领域，帮助研究者分析 MDC/CCL22 在不同实验模型（如哮喘、自身免疫疾病或肿瘤免疫调控）中的表达变化，为揭示趋化因子介导的细胞迁移与信号通路提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ccl22 ELISA Kit; Abcd1 ELISA Kit; Scya22C-C motif chemokine 22 ELISA Kit; Activated B and dendritic cell-derived ELISA Kit; CC chemokine ABCD-1 ELISA Kit; Small-inducible cytokine A22 ELISA Kit
缩写：CCL22
Uniprot No.：O88430
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell culture supernates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：23.672 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MDC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 83-92 | ||||||
| 1:2 | Average % | 100 | |||||
| Range % | 92-103 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 81-98 | ||||||
| 1:8 | Average % | 99 | |||||
| Range % | 90-103 | ||||||
回收率：
| The recovery of mouse MDC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 99 | 89-103 | |||||
| EDTA plasma (n=4) | 93 | 84-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 20002.8882.8122.8502.683 10002.5892.5132.5512.384 5002.1102.1682.1391.972 2501.4631.4461.4551.288 1250.8100.8270.8190.652 62.50.5370.5540.5460.379 31.250.3200.3110.3160.149 00.1690.1650.167 | |||||||
货期：3-5 working days
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