| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAD2L1 |
| Uniprot No | Q13257 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-205aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMALQLSREQGITLRGSAEIVAEFFSFGINS ILYQRGIYPSETFTRVQKYGLTLLVTTDLELIKYLNNVVEQLKDWLYKCS VQKLVVVISNIESGEVLERWQFDIECDKTAKDDSAPREKSQKAIQDEIRS VIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNSEE VRLRSFTTTIHKVNSMVAYKIPVND |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
nan
MAD2L1 (Mitotic Arrest Deficient 2-Like 1) is a critical component of the spindle assembly checkpoint (SAC), a surveillance mechanism ensuring accurate chromosome segregation during mitosis. This protein acts as a key sensor of unattached kinetochores, delaying anaphase onset until all chromosomes achieve proper microtubule attachment. Structurally, MAD2L1 adopts two distinct conformations: an inactive open conformation (O-MAD2) and an active closed conformation (C-MAD2), with this conformational switch being essential for SAC signaling. It interacts with checkpoint proteins like BUBR1 and CDC20 to form the mitotic checkpoint complex (MCC), which inhibits the anaphase-promoting complex/cyclosome (APC/C) and prevents premature separation of sister chromatids.
Recombinant MAD2L1 protein, typically produced in *E. coli* or insect cell expression systems, retains the functional properties of native protein and is widely used for *in vitro* studies. Its production involves cloning the MAD2L1 gene into expression vectors, followed by affinity chromatography purification (e.g., His-tag systems). The recombinant protein enables detailed investigation of SAC mechanisms, including conformational dynamics, protein-protein interactions, and phosphorylation events. Researchers employ it in biochemical assays (e.g., APC/C inhibition assays), structural studies (X-ray crystallography, NMR), and drug discovery screens targeting mitotic errors. Dysregulation of MAD2L1 is linked to chromosomal instability in cancers, making its recombinant form valuable for studying tumorigenesis and developing checkpoint-targeted therapies. Recent studies also explore its non-mitotic roles in cellular processes like DNA damage response and viral infection.
×
