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Human monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP-1/MCAF ELISA kit

  • 中文名： 人单核细胞趋化蛋白1(MCP-1/CCL2/MCAF)ELISA Kit
货号: IPD-E04655h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人单核细胞趋化蛋白1(MCP-1/CCL2/MCAF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、唾液、脑脊液（CSF）、腹水、组织培养上清液、组织匀浆.样本中的CCL2含量。CCL2 即趋化因子配体 2，是一种小分子细胞因子，属于 CC 趋化因子家族。它在免疫调节、炎症反应等过程中发挥重要作用。其研究机制主要围绕与受体 CCR2 结合，介导免疫细胞趋化，在肿瘤、神经退行性疾病等多个领域研究广泛。试剂盒检测范围为6.25 pg/ml-400 pg/ml.，灵敏度为1.56 pg/ml.。该产品适用于基础科研中探索炎症相关疾病机制、评估药物干预效果或构建疾病模型，例如在神经退行性疾病研究中分析脑脊液MCP - 1表达水平，或在肿瘤免疫微环境研究中检测细胞因子分泌动态。为体外研究MCP - 1在不同生理病理条件下的生物学功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    C-C motif chemokine 2 ELISA Kit; CCL2 ELISA Kit; CCL2_HUMAN ELISA Kit; Chemokine (C C motif) ligand 2 ELISA Kit; GDCF 2 ELISA Kit; GDCF-2 ELISA Kit; GDCF2 ELISA Kit; HC11 ELISA Kit; HSMCR30 ELISA Kit; JE ELISA Kit; MCAF ELISA Kit; MCP 1 ELISA Kit; MCP-1 ELISA Kit; MCP1 ELISA Kit; MGC9434 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic and activating factor ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; Monocyte secretory protein JE ELISA Kit; SCYA2 ELISA Kit; Small inducible cytokine A2 (monocyte chemotactic protein 1; homologous to mouse Sig je) ELISA Kit; Small inducible cytokine A2 ELISA Kit; Small inducible cytokine subfamily A (Cys Cys); member 2 ELISA Kit; Small-inducible cytokine A2 ELISA Kit; SMC CF ELISA Kit; SMC-CF ELISA Kit; SMCCF ELISA Kit
  • 缩写：
    CCL2
  • Uniprot No.：
    P13500
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, urine, saliva, cerebrospinal fluid (CSF), ascitic fluid, cell culture supernates, tissue homogenates.
  • 检测范围：
    6.25 pg/ml-400 pg/ml.
  • 灵敏度：
    1.56 pg/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human MCP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    84


    Range %

    80-90


    1:2

    Average %

    93


    Range %

    91-96


    1:4

    Average %

    90


    Range %

    88-93


    1:8

    Average %

    93


    Range %

    87-98


  • 回收率：

    The recovery of human MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    92

    89-98


    EDTA plasma (n=4)

    91

    88-96


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    400

    2.246

    2.334

    2.290

    2.169


    200

    1.552

    1.667

    1.610

    1.488


    100

    1.005

    1.028

    1.016

    0.895


    50

    0.619

    0.629

    0.624

    0.503


    25

    0.432

    0.421

    0.427

    0.305


    12.5

    0.315

    0.333

    0.324

    0.203


    6.25

    0.201

    0.194

    0.198

    0.076


    0

    0.118

    0.125

    0.121



  • 货期：
    3-5 working days

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