| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LYPLA1 |
| Uniprot No | O75608 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-230aa |
| 氨基酸序列 | MCGNNMSTPLPAIVPAARKATAAVIFLHGLGDTGHGWAEAFAGIRSSHIK YICPHAPVRPVTLNMNVAMPSWFDIIGLSPDSQEDESGIKQAAENIKALI DQEVKNGIPSNRIILGGFSQGGALSLYTALTTQQKLAGVTALSCWLPLRA SFPQGPIGGANRDISILQCHGDCDPLVPLMFGSLTVEKLKTLVNPANVTF KTYEGMMHSSCQQEMMDVKQFIDKLLPPID |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
nan
LYPLA1 (Lysophospholipase 1) is a serine hydrolase enzyme encoded by the LYPLA1 gene in humans. It belongs to the pancreatic lipase family and plays a critical role in lipid metabolism by catalyzing the hydrolysis of acyl groups from lysophospholipids, glycerides, and other lipid substrates. This enzyme is particularly known for its thioesterase activity, contributing to the regulation of fatty acid metabolism, membrane remodeling, and cellular signaling pathways. LYPLA1 is ubiquitously expressed, with significant roles in adipose tissue, liver, and the nervous system, where it influences energy homeostasis and lipid-mediated signaling.
Recombinant LYPLA1 protein is engineered for research and therapeutic applications, typically produced using expression systems like *E. coli* or mammalian cells to ensure proper folding and enzymatic activity. Purification techniques such as affinity chromatography are employed to obtain high-purity protein. Studies highlight its interaction with key metabolic regulators, including the GTPase ARFRP1. which modulates LYPLA1’s localization and activity in lipid droplets. Dysregulation of LYPLA1 has been implicated in metabolic disorders, obesity, and neurodegenerative diseases, making it a target for drug discovery.
In research, recombinant LYPLA1 facilitates enzymatic assays, inhibitor screening, and structural studies to elucidate substrate specificity and catalytic mechanisms. Its role in hydrolyzing post-translational lipid modifications (e.g., S-acylation) also links it to protein trafficking and membrane association processes. Despite progress, LYPLA1's full physiological impact and therapeutic potential remain under active investigation, particularly in metabolic syndrome and age-related pathologies.
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