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Human eosinophil chemotactic factor,ECF ELISA Kit

  • 中文名： 人嗜酸粒细胞趋化因子(ECF)ELISA Kit
货号: IPD-E04533h
Price： ￥3200
数量：
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产品详情

  • 产品描述：
    人嗜酸粒细胞趋化因子(ECF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CCL11含量。CCL11是一种趋化因子，在多种炎症反应和疾病进程中发挥作用。研究发现它能与相应受体结合，招募嗜酸性粒细胞等免疫细胞至炎症部位。深入探索其研究机制，有助于揭示疾病发病机理，为开发针对CCL11的治疗策略奠定基础。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，灵敏度为7.8 pg/mL。适用于体外研究ECF在炎症性疾病模型中的表达变化、药物干预效果评估或信号通路机制探索。血清/血浆样本可用于评估体内ECF水平动态，细胞培养上清适合监测体外刺激条件下的分泌情况，组织匀浆则便于分析局部组织中的趋化因子分布。为免疫学、病理生理学等基础研究提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    C-C motif chemokine 11 ELISA Kit; CCL 11 ELISA Kit; CCL11 ELISA Kit; CCL11_HUMAN ELISA Kit; chemokine (C-C motif) ligand 11 ELISA Kit; Chemokine C C motif ligand 11 ELISA Kit; Chemokine CC Motif Ligand 11 ELISA Kit; Chemokine ligand 11 ELISA Kit; Eosinophil chemotactic protein ELISA Kit; Eotaxin ELISA Kit; eotaxin-1 ELISA Kit; MGC22554 ELISA Kit; SCYA 11 ELISA Kit; SCYA11 ELISA Kit; Small inducible cytokine A11 ELISA Kit; Small inducible cytokine subfamily A (Cys Cys) member 11 ELISA Kit; small inducible cytokine subfamily A (Cys-Cys); member 11 (eotaxin) ELISA Kit; Small inducible cytokine subfamily A member 11 ELISA Kit; Small-inducible cytokine A11 ELISA Kit
  • 缩写：
    CCL11
  • Uniprot No.：
    P51671
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



    　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)254.273248.287251.508248.011250.633255.195SD0.0330.0300.0320.0440.0420.046CV(%)4.4304.0984.3306.0165.6986.158
  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human ECF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    89


    Range %

    85-92


    1:2

    Average %

    97


    Range %

    92-101


    1:4

    Average %

    91


    Range %

    87-94


    1:8

    Average %

    95


    Range %

    91-98









  • 回收率：

    The recovery of human ECF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    94

    89-97


    EDTA plasma (n=4)

    89

    84-92









  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.













































































    pg/ml

    OD1

    OD2

    Average

    Corrected


    2000

    2.706

    2.743

    2.725

    2.558


    1000

    1.981

    1.987

    1.984

    1.817


    500

    1.229

    1.245

    1.237

    1.070


    250

    0.745

    0.732

    0.739

    0.572


    125

    0.447

    0.454

    0.451

    0.284


    62.5

    0.323

    0.341

    0.332

    0.165


    31.25

    0.257

    0.248

    0.253

    0.086


    0

    0.168

    0.166

    0.167











  • 货期：
    3-5 working days

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