Human Carbonic Anhydrase 9(CA9) ELISA Kit

中文名： 人碳酸酐酶9(CA9)ELISA Kit

货号: IPD-E13266h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人碳酸酐酶9(CA9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CA9含量。CA9是一种靶点蛋白。它在多种肿瘤组织中高表达，与肿瘤微环境的酸化等过程相关。研究机制主要聚焦于其在肿瘤发生、发展、侵袭和转移中的作用，有望通过靶向CA9研发新型抗癌药物，为肿瘤治疗提供新策略。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，灵敏度为7.81 pg/mL。该产品适用于体外研究CA9在肿瘤发生、代谢调控或药物干预实验中的表达水平变化，为癌症机制探索、生物标志物筛选及化合物作用评估等科研领域提供可靠工具，仅限科学研究使用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
CA-IX ELISA Kit; CA9 ELISA Kit; CAH9_HUMAN ELISA Kit; CAIX ELISA Kit; Carbonate dehydratase IX ELISA Kit; Carbonic anhydrase 9 ELISA Kit; Carbonic anhydrase IX ELISA Kit; Carbonic dehydratase ELISA Kit; G250 ELISA Kit; Membrane antigen MN ELISA Kit; MN ELISA Kit; P54/58N ELISA Kit; pMW1 ELISA Kit; RCC associated protein G250 ELISA Kit; RCC-associated antigen G250 ELISA Kit; Renal cell carcinoma-associated antigen G250 ELISA Kit
缩写：
CA9
Uniprot No.：
Q16790
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
31.25 pg/mL-2000 pg/mL
灵敏度：
7.81 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cancer
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)262.027248.844253.006257.799244.127250.030SD0.0360.0400.0330.0450.0520.049CV(%)4.7625.4504.4536.0087.1636.658

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CA9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	94
	Range %	86-98
1:2	Average %	104
	Range %	98-108
1:4	Average %	102
	Range %	99-104
1:8	Average %	92
	Range %	85-96

回收率：

The recovery of human CA9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-102
EDTA plasma (n=4)	104	98-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.













pg/ml	OD1	OD2	Average	Corrected
2000	2.273	2.104	2.189	1.995
1000	1.601	1.505	1.553	1.359
500	1.111	1.057	1.084	0.890
250	0.757	0.736	0.747	0.553
125	0.484	0.475	0.480	0.286
62.5	0.351	0.342	0.347	0.153
31.25	0.263	0.256	0.260	0.066
0	0.194	0.193	0.194