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Human Carbonic anhydrase 1(CA1) ELISA kit

  • 中文名： 人碳酸酐酶1(CA1)ELISA kit
货号: IPD-EL004364HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人碳酸酐酶1(CA1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CA1含量。试剂盒检测范围为3.12 pg/mL-200 pg/mL，灵敏度为0.78 pg/mL。适用于科研领域中对CA1在肿瘤微环境、代谢调控或病理生理机制中的功能研究，也可用于探索其作为潜在生物标志物的应用价值。为体外研究CA1的生物学作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    CA 1 ELISA Kit; CA I ELISA Kit; CA-I ELISA Kit; CA1 ELISA Kit; CAB ELISA Kit; CAH1_HUMAN ELISA Kit; CAI ELISA Kit; CAN ELISA Kit; Car 1 ELISA Kit; Car1 ELISA Kit; Carbonate dehydratase I ELISA Kit; Carbonic anhydrase 1 ELISA Kit; Carbonic anhydrase A ELISA Kit; Carbonic anhydrase B ELISA Kit; Carbonic anhydrase B; formerly ELISA Kit; Carbonic anhydrase I ELISA Kit; Carbonic dehydratase ELISA Kit; ECK0125 ELISA Kit; JW0122 ELISA Kit; yadF ELISA Kit
  • 缩写：
    CA1
  • Uniprot No.：
    P00915
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human CA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    98


    Range %

    91-105


    1:2

    Average %

    87


    Range %

    83-93


    1:4

    Average %

    95


    Range %

    88-102


    1:8

    Average %

    93


    Range %

    86-98


  • 回收率：

    The recovery of human CA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    100

    96-106


    EDTA plasma (n=4)

    95

    88-101


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    200

    2.147

    2.134

    2.140

    1.959


    100

    1.588

    1.667

    1.627

    1.446


    50

    0.969

    1.028

    0.998

    0.817


    25

    0.646

    0.629

    0.637

    0.456


    12.5

    0.433

    0.421

    0.427

    0.245


    6.25

    0.311

    0.323

    0.317

    0.136


    3.12

    0.252

    0.244

    0.248

    0.067


    0

    0.178

    0.185

    0.181




  • 货期：
    3-5 working days

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