| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LYN |
| Uniprot No | P07948 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-512aa |
| 氨基酸序列 | GCIKSKGKDSLSDDGVDLKTQPVRNTERTIYVRDPTSNKQQRPVPESQLLPGQRFQTKDPEEQGDIVVALYPYDGIHPDDLSFKKGEKMKVLEEHGEWWKAKSLLTKKEGFIPSNYVAKLNTLETEEWFFKDITRKDAERQLLAPGNSAGAFLIRESETLKGSFSLSVRDFDPVHGDVIKHYKIRSLDNGGYYISPRITFPCISDMIKHYQKQADGLCRRLEKACISPKPQKPWDKDAWEIPRESIKLVKRLGAGQFGEVWMGYYNNSTKVAVKTLKPGTMSVQAFLEEANLMKTLQHDKLVRLYAVVTREEPIYIITEYMAKGSLLDFLKSDEGGKVLLPKLIDFSAQIAEGMAYIERKNYIHRDLRAANVLVSESLMCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGCFTIKSDVWSFGILLYEIVTYGKIPYPGRTNADVMTALSQGYRMPRVENCPDELYDIMKMCWKEKAEERPTFDYLQSVLDDFYTATEGQYQQQP |
| 预测分子量 | 64.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
nan
LYN, a member of the SRC family of tyrosine kinases (SFK), plays a critical role in regulating cellular signaling pathways, particularly in hematopoietic and immune cells. It exists as two major isoforms, LYN A (56 kDa) and LYN B (53 kDa), generated by alternative splicing, differing in their N-terminal regions. LYN is involved in both activating and inhibitory signaling cascades, interacting with receptors like the B-cell receptor (BCR), cytokine receptors, and integrins. It modulates key processes including cell proliferation, differentiation, adhesion, and apoptosis. Dysregulation of LYN has been implicated in various diseases, particularly cancers such as chronic lymphocytic leukemia (CLL), acute myeloid leukemia (AML), and certain solid tumors. Overactivation of LYN is associated with drug resistance and poor prognosis, while its loss-of-function mutations may contribute to autoimmune disorders.
Recombinant LYN proteins are engineered versions of the kinase produced through biotechnology methods, typically using bacterial (e.g., E. coli) or mammalian expression systems. These proteins retain functional domains, including the SH3. SH2. and kinase domains, enabling their use in biochemical and structural studies. Researchers utilize recombinant LYN to investigate kinase-substrate interactions, screen potential inhibitors for cancer therapy, and study signaling network dynamics. Its production often involves tags (e.g., GST, His-tag) for purification and detection. Recent applications include CRISPR-engineered LYN variants for functional genomics and high-throughput drug discovery platforms. As a target for precision medicine, recombinant LYN proteins have become essential tools in developing tyrosine kinase inhibitors and understanding resistance mechanisms in hematologic malignancies.
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