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| 纯度 | >98%SDS-PAGE. |
| 种属 | Human |
| 靶点 | XCL1 |
| Uniprot No | P47992 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-114aa |
| 氨基酸序列 | GSEVSDKRTC VSLTTQRLPV SRIKTYTITE GSLRAVIFIT KRGLKVCADP QATWVRDVVR SMDRKSNTRN NMIQTKPTGT QQSTNTAVTL TG |
| 预测分子量 | 10.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于XCL1重组蛋白的3篇参考文献示例(注：以下文献信息为示例，实际引用时请核实来源准确性)：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant XCL1 in a Prokaryotic System*
**作者**：Zhang Y, et al.
**摘要**：本研究在大肠杆菌中成功表达并纯化了重组XCL1蛋白，通过体外趋化实验证实其能够特异性激活表达XCR1受体的树突状细胞迁移，为后续免疫治疗研究提供基础工具。
2. **文献名称**：*Structural Insights into XCL1 Chemokine Dimerization and Receptor Binding*
**作者**：Smith J, et al.
**摘要**：通过核磁共振(NMR)解析重组XCL1蛋白的二聚体结构，发现其C端结构域对与受体XCR1的结合至关重要，揭示了XCL1在免疫信号传导中的构效关系。
3. **文献名称**：*Recombinant XCL1 Enhances Antitumor Immunity via Dendritic Cell Recruitment*
**作者**：Liu X, et al.
**摘要**：在小鼠模型中，注射重组XCL1蛋白显著增加了肿瘤微环境中CD8+ T细胞和树突状细胞的浸润，验证了其在肿瘤免疫治疗中的潜在应用价值。
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如需具体文献，建议在PubMed或Web of Science中搜索关键词“recombinant XCL1 protein”或“XCL1 expression and function”获取最新研究。
XCL1 (chemokine (C motif) ligand 1), also known as lymphotactin or SCYC1. is a unique member of the chemokine family that plays a critical role in immune regulation. Unlike typical chemokines, XCL1 adopts two distinct structural conformations—a non-canonical monomeric form (pro-XCL1) and a conserved chemokine-like fold in its mature form—enabling diverse functional interactions. It is primarily secreted by activated CD8+ T cells, natural killer (NK) cells, and γδ T cells during immune responses.
The protein binds specifically to XCR1. a G protein-coupled receptor predominantly expressed on cross-presenting dendritic cells (DCs), particularly the CD141+ (XCR1+) subset. This interaction facilitates the recruitment of antigen-presenting cells to sites of inflammation or infection, bridging innate and adaptive immunity. XCL1-XCR1 signaling is crucial for enhancing cytotoxic T cell responses, making it a focus in cancer immunotherapy and vaccine development.
Recombinant XCL1 protein is produced via bacterial or mammalian expression systems, often requiring refolding or post-translational modifications to ensure bioactivity. Its applications span immunological research, including studies on T cell-DC crosstalk, antitumor immunity, and inflammatory diseases. In therapeutic contexts, XCL1 has been explored as an adjuvant to boost DC recruitment in vaccines or to modulate immune microenvironments in tumors.
Despite its protective roles, dysregulated XCL1 expression is linked to chronic inflammation and autoimmune disorders, highlighting its dual nature in immune homeostasis. Ongoing research aims to harness its chemotactic properties while mitigating pathological effects, positioning recombinant XCL1 as a valuable tool for both mechanistic studies and translational immunology.
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