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Human β2-microglobulin,BMG/β2-MG ELISA Kit

  • 中文名: 人β2微球蛋白(BMG/β2-MG)ELISA Kit
货号: IPD-E04883h
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人β2微球蛋白(BMG/β2-MG)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、尿液样本中的B2M含量。B2M即β2-微球蛋白,它是MHCⅠ类分子的轻链,广泛存在于有核细胞表面。在肿瘤免疫等研究机制中,B2M参与抗原呈递过程。其表达异常与多种疾病相关,如肿瘤免疫逃逸等,深入研究有助于理解疾病发病机制并为治疗提供新靶点。试剂盒检测范围为0.039 μg/mL-10 μg/mL,灵敏度为0.039 μg/mL。适用于科研场景中对β2 - MG的动态监测,如探索肾脏疾病进展机制、评估肿瘤微环境中免疫调节作用,或研究自身免疫性疾病中细胞代谢异常等方向本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    B2M ELISA Kit; B2MG_HUMAN ELISA Kit; Beta 2 microglobin ELISA Kit; Beta 2 microglobulin ELISA Kit; Beta 2 microglobulin precursor ELISA Kit; Beta chain of mhc class 1 proteins ELISA Kit; Beta chain of MHC class I molecules ELISA Kit; Beta-2-microglobulin form pI 5.3 ELISA Kit; CDABP0092 ELISA Kit; Hdcma22p ELISA Kit; IMD43 ELISA Kit
  • 缩写:
    B2M
  • Uniprot No.:
    P61769
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, urine
  • 检测范围:
    0.039 μg/mL-10 μg/mL
  • 灵敏度:
    0.039 μg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human β2-MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    86


    Range %

    81-92


    1:2

    Average %

    85


    Range %

    80-92


    1:4

    Average %

    87


    Range %

    81-96


    1:8

    Average %

    89


    Range %

    84-95


  • 回收率:

    The recovery of human β2-MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    91

    87-95


    EDTA plasma (n=4)

    87

    83-96


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    μg/ml

    OD1

    OD2

    Average




    10

    0.315

    0.310

    0.312




    2.5

    0.716

    0.722

    0.719




    0.625

    1.174

    1.128

    1.151




    0.156

    1.648

    1.629

    1.638




    0.039

    1.938

    1.821

    1.880




    0

    2.066

    2.183

    2.125





  • 货期:
    3-5 working days

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