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Mouse Arginase-1(ARG1) ELISA kit

  • 中文名： 小鼠精氨酸酶1(ARG1)ELISA kit
货号: IPD-EL002005MO
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    小鼠精氨酸酶1(ARG1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ARG1含量。小鼠精氨酸酶1（ARG1）是一种在巨噬细胞中表达的酶，参与调节小鼠的发育和大脑功能。其机制涉及调节精氨酸代谢，影响胆碱能神经支配和神经元突触可塑性，进而影响认知行为。研究显示，ARG1在小胶质细胞中具有独特的分子特征，对大脑发育和功能具有重要作用。试剂盒检测范围为2.7 pg/ml-2000 pg/ml，灵敏度为2.7 pg/ml。适用于细胞实验、动物模型等科研场景中动态监测ARG1表达变化，例如探究肝脏代谢异常、免疫细胞功能调控或药物干预对精氨酸代谢通路的影响本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Arg1Arginase-1 ELISA kit; EC 3.5.3.1 ELISA kit; Liver-type arginase ELISA kit; Type I arginase ELISA kit
  • 缩写：
    ARG1
  • Uniprot No.：
    Q61176
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    2.7 pg/ml-2000 pg/ml.
  • 灵敏度：
    2.7 pg/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ARG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:100

    Average %

    84


    Range %

    80-92


    1:200

    Average %

    97


    Range %

    91-105


    1:400

    Average %

    97


    Range %

    92-110


    1:800

    Average %

    93


    Range %

    86-98


  • 回收率：

    The recovery of mouse ARG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    89

    82-96


    EDTA plasma (n=4)

    97

    92-103


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    2000

    1.897

    1.934

    1.916

    1.732


    666

    1.238

    1.327

    1.282

    1.098


    222

    0.768

    0.818

    0.793

    0.609


    74

    0.509

    0.529

    0.519

    0.335


    24.6

    0.419

    0.401

    0.410

    0.226


    8.2

    0.320

    0.343

    0.332

    0.148


    2.7

    0.256

    0.264

    0.260

    0.076


    0

    0.183

    0.185

    0.184



  • 本试剂盒所含材料：


    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse ARG1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled ARG1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料：



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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