首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠载脂蛋白E(Apo-E)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的APOE含量。APOE（载脂蛋白E）是一种脂质载体，对胆固醇平衡至关重要。其基因多态性导致三种同工型（E2、E3、E4）表达。APOE4等位基因与阿尔茨海默病风险增加相关，通过调节大脑中β淀粉样蛋白的聚集和清除，影响脑脂质转运、糖代谢、神经元信号和神经炎症。试剂盒检测范围为6.25 ng/mL-400 ng/mL，灵敏度为1.56 ng/mL。该产品可广泛应用于代谢调控机制研究、动脉粥样硬化模型评估、阿尔茨海默病相关动物实验等科研领域，为探索Apo - E在疾病发生发展中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ApoeApolipoprotein E ELISA kit; Apo-E ELISA kit
缩写：APOE
Uniprot No.：P08226
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 99 | ||||
| Range % | 96-101 | |||||
| 1:200 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:400 | Average % | 100 | ||||
| Range % | 97-103 | |||||
| 1:800 | Average % | 83 | ||||
| Range % | 80-86 | |||||
回收率：
| The recovery of mouse Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 93-100 | ||||
| EDTA plasma (n=4) | 99 | 95-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 4000.1570.1590.158 2000.2490.2690.259 1000.4320.4410.437 500.6430.6720.658 250.9550.9840.970 12.51.1991.1721.186 6.251.5681.5721.570 02.5732.5912.582 | ||||||
本试剂盒所含材料：
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A 96-well Assay plate --The 96-well plate has been pre-coated with ApoE.
Two pieces of Standard (Freeze-dried)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
HRP-conjugated ApoE antibody (100 x concentrate) (1 x 6 μl) --Act as the detection antibody.
HRP-conjugate Diluent (1 x 10 ml) --Dilute the HRP-conjugated ApoE antibody solution.
Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
本试剂盒不含材料：
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A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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