首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人载脂蛋白E(Apo-E)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的APOE含量。APOE基因编码载脂蛋白E，在脂质代谢等过程中发挥关键作用。其表达产物参与脂蛋白的形成、转运和代谢。研究发现，APOE基因的不同亚型与心血管疾病、阿尔茨海默病等的发生发展相关，通过影响脂质代谢和蛋白聚集等机制参与疾病进程。试剂盒检测范围为15.62 ng/mL-1000 ng/mL，灵敏度为3.9 ng/mL。该产品适用于心血管疾病模型、神经退行性疾病研究、代谢综合征机制探索等科研领域，支持多种生物样本类型分析，为体外实验提供可靠的Apo - E浓度定量数据，助力脂质代谢调控及相关疾病病理学的分子机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AD2 ELISA Kit; Apo-E ELISA Kit; APOE ELISA Kit; APOE_HUMAN ELISA Kit; APOEA ELISA Kit; Apolipoprotein E ELISA Kit; Apolipoprotein E3 ELISA Kit; ApolipoproteinE ELISA Kit; Apoprotein ELISA Kit; LDLCQ5 ELISA Kit; LPG ELISA Kit
缩写：APOE
Uniprot No.：P02649
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.62 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:200 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:400 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:800 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 10000.0880.0900.089 5000.1350.1420.139 2500.2270.2370.232 1250.3240.3410.333 62.50.5830.5980.591 31.250.8470.8640.856 15.621.2281.2351.232 02.1552.1992.177 | ||||||
货期：3-5 working days
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