首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：兔载脂蛋白B100(Apo-B100)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Apo-B100含量。Apo-B100是低密度脂蛋白（LDL）的主要结构蛋白，参与脂质和胆固醇代谢，与动脉粥样硬化密切相关。研究显示，Apo-B100通过冷冻电镜、AlphaFold2和分子动力学方法解析其结构，揭示其与LDL受体（LDLR）的相互作用机制，为开发针对胆固醇代谢异常的新疗法提供新视角。试剂盒检测范围为78 ng/mL-5000 ng/mL，灵敏度为19.5 ng/mL。适用于基础科研中脂代谢机制研究、动脉粥样硬化病理模型分析、药物干预对脂蛋白合成影响的评估等方向，为心血管疾病相关动物实验提供可靠的检测工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：APOBApolipoprotein B ELISA kit; Fragment ELISA kit
缩写：Apo-B100
Uniprot No.：P17165
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：78 ng/mL-5000 ng/mL
灵敏度：19.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:200 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:400 | Average % | 104 | ||||
| Range % | 98-108 | |||||
| 1:800 | Average % | 85 | ||||
| Range % | 80-89 | |||||
回收率：
| The recovery of rabbit Apo-B100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 93-102 | ||||
| EDTA plasma (n=4) | 87 | 82-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 50000.0920.0910.092 25000.1460.1380.142 12500.2460.2360.241 6250.3750.3600.368 3120.5240.5390.532 1560.9340.8770.906 781.4471.3341.391 02.4322.5622.497 | ||||||
货期：3-5 working days
×
