Human Angiopoietin 2,ANG-2 ELISA Kit

中文名： 人血管生成素2(ANG-2)ELISA Kit

货号: IPD-E04500h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人血管生成素2(ANG-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ANGPT2含量。ANGPT2是一种重要靶点。它在血管生成、血管稳定等生理过程中发挥关键作用。研究发现其可调节血管内皮细胞功能，与肿瘤生长、转移及眼部新生血管疾病等病理过程密切相关，对其作用机制的深入探索有望为相关疾病治疗提供新思路。试剂盒检测范围为39 pg/mL-2500 pg/mL，灵敏度为9.75 pg/mL。适用于心血管疾病机制研究、肿瘤血管生成分析、炎症相关动物模型评估等科研领域，尤其适用于需要多维度样本对比的转化医学研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
AGPT 2 ELISA Kit; Agpt2 ELISA Kit; ANG 2 ELISA Kit; ANG-2 ELISA Kit; ANG2 ELISA Kit; Angiopoietin 2a ELISA Kit; Angiopoietin 2B ELISA Kit; Angiopoietin-2 ELISA Kit; Angiopoietin2 ELISA Kit; ANGP2_HUMAN ELISA Kit; ANGPT 2 ELISA Kit; Angpt2 ELISA Kit; Tie2 ligand ELISA Kit
缩写：
ANGPT2
Uniprot No.：
O15123
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
39 pg/mL-2500 pg/mL
灵敏度：
9.75 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)312.245312.541312.837312.659312.866312.837SD0.0330.0320.0280.0420.0460.043CV(%)3.9053.7833.3064.9625.4305.077

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ANG-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

83-96

1:2

Average %

Range %

91-98

1:4

Average %

Range %

88-101

1:8

Average %

Range %

91-103

回收率：

The recovery of human ANG-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-92
EDTA plasma (n=4)	95	92-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.











pg/ml	OD1	OD2	Average	Corrected
2500	2.925	2.960	2.943	2.784
1250	2.547	2.505	2.526	2.367
625	1.656	1.799	1.728	1.569
312.5	0.829	0.888	0.859	0.700
156.25	0.401	0.411	0.406	0.247
78	0.253	0.252	0.253	0.094
39	0.211	0.235	0.223	0.064
0	0.165	0.152	0.159