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Human Receptor for advanced glycation end products, RAGE/AGER ELISA Kit

  • 中文名： 人晚期糖基化终产物特异性受体(RAGE/AGER)ELISA Kit
货号: IPD-E09354h
Price： ￥2500
数量：
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产品详情

  • 产品描述：人晚期糖基化终产物特异性受体酶联免疫试剂盒 检测血清、血浆、组织匀浆样本中的AGER含量。AGER即晚期糖基化终末产物受体，在多种生理和病理过程中发挥作用。它参与炎症反应、氧化应激等机制。研究发现AGER与糖尿病并发症、神经退行性疾病等密切相关，其信号通路的调控机制是当前研究热点，有望为相关疾病治疗提供新靶点。试剂盒检测范围为78 pg/mL-5000 pg/ml，灵敏度为19.5 pg/mL。该产品适用于体外定量分析不同生物样本中RAGE的表达水平，可为糖尿病相关机制研究、心血管疾病模型构建或神经退行性病变的分子通路探索提供可靠数据支持，满足科研领域对RAGE蛋白检测的高特异性需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：Advanced glycosylation end product-specific receptor ELISA Kit; Ager ELISA Kit; DAMA 358M23.4 ELISA Kit; MGC2235 ELISA Kit; MGC22357 ELISA Kit; RAGE_HUMAN ELISA Kit; Receptor for advanced glycation end products ELISA Kit; Receptor for advanced glycosylation end products ELISA Kit

  • 缩写：AGER

  • Uniprot No.：Q15109

  • 种属：Homo sapiens (Human)

  • 样本类型：serum, plasma, tissue homogenates

  • 检测范围：78 pg/mL-5000 pg/mL

  • 灵敏度：19.5 pg/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Immunology

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human AGER in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.



    		SampleSerum(n=4)
    1:1Average %88
    Range %80-93

    1:2Average %92
    Range %88-96

    1:4Average %89
    Range %85-96

    1:8Average %93
    Range %90-97

  • 回收率：

    The recovery of human AGER spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9287-96
    EDTA plasma (n=4)9591-99














  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected 50002.7022.7192.7112.512 25002.0112.0622.0371.838 12501.4031.4211.4121.213 6250.9280.9390.9340.735 3120.5710.5810.5760.377 1560.3860.3960.3910.192 780.2750.2620.2690.070 00.1970.2000.199　 






  • 货期：3-5 working days

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