Mouse Alpha-fetoprotein,AFP ELISA Kit

中文名： 小鼠甲种胎儿球蛋白/甲胎蛋白(AFP)ELISA Kit

货号: IPD-E08282m

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
小鼠甲种胎儿球蛋白/甲胎蛋白(AFP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的AFP含量。AFP即甲胎蛋白，是一种糖蛋白。其正常情况下主要由胎儿肝细胞及卵黄囊合成。在成人中，AFP升高常见于肝癌等恶性肿瘤。研究上常将其作为肿瘤标志物，通过检测其水平辅助肝癌等疾病的诊断、监测及预后评估。试剂盒检测范围为1.23 ng/ml-900 ng/ml.，灵敏度为1.23 ng/ml.。科研人员可通过探究肝损伤修复、肿瘤发生发展或胚胎发育相关机制，为构建疾病模型、评估干预效果提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
AfpAlpha-fetoprotein ELISA kit; Alpha-1-fetoprotein ELISA kit; Alpha-fetoglobulin ELISA kit
缩写：
AFP
Uniprot No.：
P02772
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates.
检测范围：
1.23 ng/ml-900 ng/ml.
灵敏度：
1.23 ng/ml.
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cancer
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)34.05634.84635.72033.81934.25334.648SD0.0350.0380.0420.0360.0420.053CV(%)4.4814.7445.1034.6455.3446.658

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

82-93

1:2

Average %

Range %

94-104

1:4

Average %

Range %

96-108

1:8

Average %

Range %

80-100

回收率：

The recovery of mouse AFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	83-97
EDTA plasma (n=4)	96	92-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
900	2.379	2.252	2.316	2.161
300	1.975	1.876	1.926	1.771
100	1.305	1.304	1.305	1.150
33.33	0.812	0.779	0.796	0.641
11.11	0.534	0.494	0.514	0.359
3.7	0.372	0.349	0.361	0.206
1.23	0.264	0.249	0.257	0.102
0	0.161	0.149	0.155