| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LMX1B |
| Uniprot No | O60663-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-395aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMDIATGPESLERCFPRGQTDCAKMLDG IKMEEHALRPGPATLGVLLGSDCPHPAVCEGCQRPISDRFLMRVNESSWH EECLQCAACQQALTTSCYFRDRKLYCKQDYQQLFAAKCSGCMEKIAPTEF VMRALECVYHLGCFCCCVCERQLRKGDEFVLKEGQLLCKGDYEKEKDLLS SVSPDESDSVKSEDEDGDMKPAKGQGSQSKGSGDDGKDPRRPKRPRTILT TQQRRAFKASFEVSSKPCRKVRETLAAETGLSVRVVQVWFQNQRAKMKKL ARRHQQQQEQQNSQRLGQEVLSSRMEGMMASYTPLAPPQQQIVAMEQSPY GSSDPFQQGLTPPQMPGNDSIFHDIDSDTSLTSLSDCFLGSSDVGSLQAR VGNPIDRLYSMQSSYFAS |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LMX1B重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Recombinant LMX1B Protein Synthesis and Its Role in Podocyte Differentiation"*
**作者**: Smith A, et al.
**摘要**: 研究描述了通过大肠杆菌表达系统制备重组LMX1B蛋白，并验证其在体外促进肾小球足细胞分化的功能。结果显示，重组LMX1B通过激活WT1等靶基因调控足细胞发育。
2. **文献名称**: *"DNA Binding Properties of Recombinant LMX1B: Implications for Nail-Patella Syndrome"*
**作者**: Chen L, et al.
**摘要**: 通过体外表达纯化LMX1B蛋白，研究其与COL4A3/COL4A4基因启动子的结合能力，发现部分突变型LMX1B(如R246Q)导致DNA结合活性丧失，解释了Nail-Patella综合征的分子机制。
3. **文献名称**: *"LMX1B Recombinant Protein Rescues Dopaminergic Neuron Development in Zebrafish Models"*
**作者**: Kim H, et al.
**摘要**: 利用昆虫细胞系统表达功能性LMX1B重组蛋白，并在斑马鱼模型中验证其修复多巴胺能神经元发育缺陷的作用，为神经发育障碍治疗提供实验依据。
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注：以上文献为示例，实际引用时需核实具体来源及细节。如需真实文献，建议通过PubMed或Web of Science以“LMX1B recombinant protein”为关键词检索近年研究。
LMX1B is a LIM-homeodomain transcription factor critical during embryonic development, particularly in the specification of dorsal-ventral patterning in the central nervous system, limbs, and kidneys. It regulates the expression of genes involved in cell differentiation, tissue morphogenesis, and organogenesis. Mutations in the *LMX1B* gene are associated with Nail-Patella syndrome (NPS), an autosomal dominant disorder characterized by skeletal abnormalities, nail dysplasia, and renal defects.
Recombinant LMX1B protein refers to the engineered form of LMX1B produced in vitro using expression systems like *E. coli* or mammalian cell cultures. This allows researchers to obtain purified, functional protein for mechanistic studies. The recombinant protein retains key structural domains: two N-terminal LIM domains (mediating protein-protein interactions) and a C-terminal homeodomain (essential for DNA binding).
In research, recombinant LMX1B is utilized to investigate its role in transcriptional regulation, interaction with co-factors (e.g., LDB1), and signaling pathways (e.g., Wnt/β-catenin). It also aids in modeling NPS-related mutations to dissect their impact on protein function. Additionally, recombinant LMX1B has potential therapeutic applications, such as in vitro differentiation protocols for generating dopaminergic neurons, leveraging its role in midbrain development.
Current challenges include optimizing protein stability and post-translational modifications to mimic native conditions. Advances in recombinant technology continue to enhance its utility in both basic research and translational studies targeting developmental disorders linked to LMX1B dysregulation.
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