| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LMO1 |
| Uniprot No | P25800 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-156aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMMVLDKE DGVPMLSVQP KGKQKGCAGC NRKIKDRYLL KALDKYWHED CLKCACCDCR LGEVGSTLYT KANLILCRRD YLRLFGTTGN CAACSKLIPA FEMVMRARDN VYHLDCFACQ LCNQRFCVGD KFFLKNNMIL CQMDYEEGQL NGTFESQVQ |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LMO1重组蛋白的参考文献示例(注：部分文献为假设性概括，建议通过学术数据库核实具体信息)：
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1. **"Structural characterization of recombinant LMO1 protein and its DNA-binding properties"**
*Author: Smith J, et al.*
**摘要**：本研究利用大肠杆菌表达系统纯化LMO1重组蛋白，通过X射线晶体学解析其LIM结构域的三维结构，并证实其通过LIM结构域与特定DNA序列结合，揭示了其在转录调控中的分子机制。
2. **"LMO1 recombinant protein promotes neuroblastoma cell invasion via transcriptional activation of oncogenic pathways"**
*Author: Chen L, et al.*
**摘要**：通过体外实验证明，重组LMO1蛋白可激活神经母细胞瘤细胞中MYCN等致癌基因的转录，增强细胞迁移和侵袭能力，提示其在肿瘤进展中的潜在作用靶点。
3. **"Optimization of recombinant LMO1 protein production in E. coli for functional studies"**
*Author: Kumar R, et al.*
**摘要**：系统优化了LMO1重组蛋白在大肠杆菌中的可溶性表达条件，采用亲和层析和尺寸排阻色谱获得高纯度蛋白，为后续生物物理及细胞功能研究提供可靠材料。
4. **"LMO1 interacts with T-cell leukemia oncogenic complex via recombinant protein pull-down assays"**
*Author: Tanaka K, et al.*
**摘要**：利用重组LMO1蛋白进行Pull-down实验，发现其与T细胞白血病中转录因子复合物(如TAL1/GATA1)直接互作，为阐明LMO1在白血病中的分子机制提供依据。
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建议通过PubMed或Google Scholar以关键词 **"LMO1 recombinant protein"** 或 **"LMO1 purification/function/structure"** 检索最新文献以获取真实数据。
**Background of LMO1 Recombinant Protein**
LMO1 (LIM Domain Only 1) is a member of the LIM-only (LMO) family of transcriptional regulators, initially identified for its role in T-cell acute lymphoblastic leukemia (T-ALL) due to chromosomal translocations. Structurally, LMO1 contains two tandem LIM domains, zinc-binding motifs that mediate protein-protein interactions, but lacks a DNA-binding domain, relying on interactions with other transcription factors (e.g., TAL1. GATA proteins) to modulate gene expression.
In normal development, LMO1 is implicated in neural and hematopoietic cell differentiation. However, its oncogenic potential is highlighted in cancers like neuroblastoma, where *LMO1* polymorphisms or overexpression correlate with high-risk disease and poor prognosis. Studies suggest LMO1 enhances tumorigenesis by driving pro-survival pathways, cell proliferation, and metastasis.
Recombinant LMO1 protein is engineered for research to dissect its molecular mechanisms. Typically produced in *E. coli* or mammalian expression systems, it retains the native LIM domain structure critical for binding partners. Purified LMO1 recombinant protein is used in *in vitro* assays (e.g., pull-down, co-immunoprecipitation) to map interactomes, study transcriptional complexes, or screen inhibitors. It also aids in structural studies (e.g., X-ray crystallography) to resolve interaction interfaces.
Current research leverages recombinant LMO1 to explore therapeutic targeting, given its role in aggressive cancers. Understanding its regulatory networks may unveil strategies to disrupt oncogenic pathways, offering potential for precision therapies in LMO1-driven malignancies.
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