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Rabbit Polyclonal PXN Antibody

  • 中文名： PXN抗体
  • 别    名： nan
货号: IPDX04165
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/30-1/150 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

WB Predicted band size65 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenSynthetic peptide of human PXN
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于PXN(Paxillin)抗体的3篇参考文献示例，包含文献名称、作者及摘要概括：

1. **"Paxillin: A focal adhesion-associated adaptor protein"**

*作者：Turner CE, Brown MC*

摘要：该综述探讨了Paxillin在粘着斑中的核心作用，重点描述了其作为支架蛋白在整合细胞外基质信号与细胞内信号通路(如细胞迁移和增殖)中的功能。文献提及使用特异性PXN抗体进行免疫共沉淀和免疫荧光实验，验证Paxillin与多种信号分子(如FAK、Src)的相互作用及亚细胞定位。

2. **"Paxillin phosphorylation in regulating cell motility and cancer progression"**

*作者：Deakin NO, Turner CE*

摘要：研究聚焦于Paxillin磷酸化修饰(如Tyr31/Ser83位点)在肿瘤细胞迁移和侵袭中的作用。通过Western blot和免疫荧光技术，采用磷酸化特异性PXN抗体，揭示磷酸化Paxillin与肿瘤转移的相关性，并探讨其作为癌症治疗靶点的潜力。

3. **"Role of Paxillin in hepatocellular carcinoma: Overexpression correlates with poor prognosis"**

*作者：Huang C, Chen J*

摘要：该临床研究利用PXN抗体进行免疫组织化学染色，分析肝细胞癌(HCC)组织中Paxillin的表达水平，发现其过表达与肿瘤大小、转移及患者生存率降低显著相关，提示Paxillin可作为HCC预后的生物标志物。

(注：以上文献信息为示例性概括，实际引用需以具体出版物为准。)

背景信息

Paxillin (PXN) is a multifunctional adaptor protein primarily involved in cell adhesion, migration, and signaling. Discovered in the early 1990s, it localizes to focal adhesions, dynamic structures that mediate interactions between the extracellular matrix (ECM) and the actin cytoskeleton. PXN serves as a scaffold, integrating signals from integrins, growth factor receptors, and cytoskeletal regulators. Its structure includes LD motifs and LIM domains, which facilitate binding to kinases (e.g., FAK, Src), phosphatases, and other adhesion-related proteins, enabling coordination of cell motility and survival pathways. Dysregulation of PXN expression or phosphorylation is linked to cancer metastasis, fibrosis, and cardiovascular diseases, making it a biomarker for pathological processes. PXN antibodies are essential tools for studying these mechanisms. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess PXN expression, post-translational modifications (e.g., tyrosine phosphorylation at Y31/Y118), and subcellular localization. Commercial PXN antibodies vary in specificity (e.g., targeting N-terminal vs. C-terminal regions) and applications, requiring validation for experimental contexts. Recent research explores PXN's role in mechanotransduction and its potential as a therapeutic target in invasive cancers, driving demand for high-quality, well-characterized antibodies.

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