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| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANGPT1 |
| Uniprot No | Q15389 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-480aa |
| 氨基酸序列 | MSNQRRSPENSGRRYNRIQHGQCAYTFILPEHDGNCRESTTDQYNTNALQ RDAPHVEPDFSSQKLQHLEHVMENYTQWLQKLENYIVENMKSEMAQIQQN AVQNHTATMLEIGTSLLSQTAEQTRKLTDVETQVLNQTSRLEIQLLENSL STYKLEKQLLQQTNEILKIHEKNSLLEHKILEMEGKHKEELDTLKEEKEN LQGLVTRQTYIIQELEKQLNRATTNNSVLQKQQLELMDTVHNLVNLCTKE GVLLKGGKREEEKPFRDCADVYQAGFNKSGIYTIYINNMPEPKKVFCNMD VNGGGWTVIQHREDGSLDFQRGWKEYKMGFGNPSGEYWLGNEFIFAITSQ RQYMLRIELMDWEGNRAYSQYDRFHIGNEKQNYRLYLKGHTGTAGKQSSL ILHGADFSTKDADNDNCMCKCALMLTGGWWFDACGPSNLNGMFYTAGQNH GKLNGIKWHYFKGPSYSLRSTTMMIRPLDF |
| 预测分子量 | 56 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANGPT1重组蛋白的3篇参考文献示例(内容为虚构，仅作格式参考)：
1. **文献名称**：Recombinant ANGPT1 Protein Enhances Vascular Stability in Sepsis Models
**作者**：Kim H, et al.
**摘要**：本研究利用哺乳动物细胞表达系统制备了高纯度重组ANGPT1蛋白，并在脓毒症小鼠模型中验证其功能。实验表明，重组ANGPT1通过激活Tie2受体减少血管渗漏，显著降低炎症因子水平，提示其潜在治疗价值。
2. **文献名称**：ANGPT1 Gene Delivery Promotes Cardiac Repair via Angiogenesis in Myocardial Infarction
**作者**：Chen L, et al.
**摘要**：通过腺病毒载体递送重组ANGPT1至心肌梗死大鼠心脏，发现其促进新生血管形成并减少纤维化，改善心功能。研究证明ANGPT1通过协同VEGF增强内皮细胞迁移和管腔形成。
3. **文献名称**：A Novel Bacterial Expression System for Cost-effective ANGPT1 Production
**作者**：Wang Y, et al.
**摘要**：开发了一种基于大肠杆菌的重组ANGPT1表达纯化工艺，通过分子伴侣共表达解决包涵体问题。获得的蛋白在体外实验中成功激活下游PI3K/Akt通路，为大规模生产提供经济方案。
注：以上为模拟内容，实际文献需通过PubMed、Web of Science等数据库检索关键词“recombinant ANGPT1 protein”获取。
ANGPT1 (Angiopoietin-1) is a secreted glycoprotein that plays a critical role in vascular development and homeostasis. It belongs to the angiopoietin family and primarily binds to the Tie2 receptor tyrosine kinase expressed on endothelial cells. Unlike its homolog ANGPT2. which acts as a context-dependent agonist/antagonist, ANGPT1 consistently promotes Tie2 phosphorylation, stabilizing blood vessels by enhancing endothelial cell survival, reducing vascular permeability, and supporting interactions between endothelial and perivascular cells. This protein is essential during embryogenesis for cardiovascular development and continues to regulate vascular quiescence in adults.
Recombinant ANGPT1 proteins are engineered using mammalian or bacterial expression systems to mimic native protein activity. These products typically include the fibrinogen-like domain responsible for receptor binding and oligomerization. Researchers utilize recombinant ANGPT1 to study vascular biology, particularly in contexts like angiogenesis, vascular maturation, and inflammatory responses. Its therapeutic potential has been explored in preclinical models for conditions involving vascular dysfunction, including acute lung injury, myocardial infarction, and diabetic retinopathy. Notably, modified forms like COMP-Angpt1 (a soluble, stable variant) show enhanced efficacy in reducing vascular leakage and inflammation compared to native ANGPT1.
Current challenges in therapeutic applications involve optimizing delivery methods and understanding context-specific effects, as excessive angiogenesis promotion could theoretically support tumor growth. Nevertheless, recombinant ANGPT1 remains a valuable tool for deciphering vascular signaling pathways and developing regenerative therapies targeting endothelial stabilization.
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