| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KPNB1 |
| Uniprot No | Q14974 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-876aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMELITIL EKTVSPDRLE LEAAQKFLER AAVENLPTFL VELSRVLANP GNSQVARVAA GLQIKNSLTS KDPDIKAQYQ QRWLAIDANA RREVKNYVLQ TLGTETYRPS SASQCVAGIA CAEIPVNQWP ELIPQLVANV TNPNSTEHMK ESTLEAIGYI CQDIDPEQLQ DKSNEILTAI IQGMRKEEPS NNVKLAATNA LLNSLEFTKA NFDKESERHF IMQVVCEATQ CPDTRVRVAA LQNLVKIMSL YYQYMETYMG PALFAITIEA MKSDIDEVAL QGIEFWSNVC DEEMDLAIEA SEAAEQGRPP EHTSKFYAKG ALQYLVPILT QTLTKQDEND DDDDWNPCKA AGVCLMLLAT CCEDDIVPHV LPFIKEHIKN PDWRYRDAAV MAFGCILEGP EPSQLKPLVI QAMPTLIELM KDPSVVVRDT AAWTVGRICE LLPEAAINDV YLAPLLQCLI EGLSAEPRVA SNVCWAFSSL AEAAYEAADV ADDQEEPATY CLSSSFELIV QKLLETTDRP DGHQNNLRSS AYESLMEIVK NSAKDCYPAV QKTTLVIMER LQQVLQMESH IQSTSDRIQF NDLQSLLCAT LQNVLRKVQH QDALQISDVV MASLLRMFQS TAGSGGVQED ALMAVSTLVE VLGGEFLKYM EAFKPFLGIG LKNYAEYQVC LAAVGLVGDL CRALQSNIIP FCDEVMQLLL ENLGNENVHR SVKPQILSVF GDIALAIGGE FKKYLEVVLN TLQQASQAQV DKSDYDMVDY LNELRESCLE AYTGIVQGLK GDQENVHPDV MLVQPRVEFI LSFIDHIAGD EDHTDGVVAC AAGLIGDLCT AFGKDVLKLV EARPMIHELL TEGRRSKTNK AKTLATWATK ELRKLKNQA |
| 预测分子量 | 100 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于KPNB1重组蛋白的关键文献概览(虚构示例，仅供参考)：
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1. **文献名称**: *Structural insights into KPNB1-mediated nuclear import of proteins*
**作者**: Smith A, et al. (2018)
**摘要**: 通过重组表达人源KPNB1蛋白并解析其晶体结构，揭示其与核定位信号(NLS)结合的分子机制，为设计靶向核转运的小分子抑制剂提供结构基础。
2. **文献名称**: *KPNB1 overexpression promotes cancer cell proliferation via regulating p53 nuclear translocation*
**作者**: Chen L, et al. (2020)
**摘要**: 研究利用重组KPNB1蛋白验证其在癌细胞中的功能，发现其通过调控p53蛋白的核质转运促进肿瘤生长，提示KPNB1作为癌症治疗靶点的潜力。
3. **文献名称**: *In vitro reconstitution of KPNB1-dependent nuclear import using recombinant proteins*
**作者**: Tanaka R, et al. (2019)
**摘要**: 建立基于重组KPNB1和RanGTP的体外核转运实验体系，证明KPNB1在介导大分子复合物跨核膜运输中的必要性，并开发了高通量筛选核转运抑制剂的方法。
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*注：以上文献信息为模拟生成，实际研究中请通过PubMed或Web of Science等平台检索真实文献。*
KPNB1 (Karyopherin Subunit Beta 1), also known as importin β1. is a key member of the importin β family of nuclear transport receptors. It plays a central role in nucleocytoplasmic transport by mediating the translocation of proteins and certain RNAs across the nuclear envelope. KPNB1 typically functions by binding to importin α, which recognizes cargo proteins containing a classical nuclear localization signal (NLS). Together, the KPNB1-importin α complex facilitates cargo delivery into the nucleus through interactions with the nuclear pore complex. Beyond classical NLS-dependent transport, KPNB1 also participates in importin α-independent transport of specific cargoes, such as ribosomal proteins and viral components.
Structurally, KPNB1 contains multiple HEAT repeats that form a flexible superhelical structure, enabling dynamic interactions with diverse binding partners. This adaptability allows it to regulate critical cellular processes, including cell cycle progression, transcriptional regulation, and stress responses. Dysregulation of KPNB1 has been linked to diseases such as cancer, neurodegenerative disorders, and viral infections, making it a potential therapeutic target.
Recombinant KPNB1 protein is widely used in studies exploring nuclear transport mechanisms, protein-protein interactions, and drug discovery. It is commonly expressed in *E. coli* or mammalian systems, purified via affinity tags (e.g., His-tag), and validated for functionality through nuclear import assays or binding studies. Researchers leverage this tool to investigate inhibitors targeting KPNB1’s interaction with viral proteins or oncogenic cargoes, aiming to develop antiviral or anticancer therapies. Its role in cellular homeostasis and disease underscores its importance in both basic research and translational applications.
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