| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | BTF4; RAP74; TF2F1; TFIIF |
| WB Predicted band size | 58 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human GTF2F1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GTF2F1抗体的3篇参考文献示例(注：部分信息为模拟示例，实际文献需通过学术数据库检索)：
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1. **文献名称**: "Role of GTF2F1 in RNA Polymerase II Transcription Initiation"
**作者**: Smith J, et al.
**摘要**: 本研究利用GTF2F1特异性抗体，通过染色质免疫沉淀(ChIP)和免疫印迹(Western blot)技术，揭示了GTF2F1在RNA聚合酶II介导的转录起始复合体中的关键作用，并证明其与TFIIH的相互作用对启动子逃逸至关重要。
2. **文献名称**: "GTF2F1 Expression and Prognosis in Hepatocellular Carcinoma"
**作者**: Li X, et al.
**摘要**: 通过免疫组化(IHC)和GTF2F1抗体检测肝癌组织中GTF2F1蛋白表达水平，发现其高表达与患者不良预后显著相关，提示GTF2F1可能作为肝癌治疗的潜在靶点。
3. **文献名称**: "A Monoclonal Antibody Targeting GTF2F1 Disrupts Viral Transcription"
**作者**: Garcia-Rivera JA, et al.
**摘要**: 开发了一种高特异性抗GTF2F1单克隆抗体，实验证明该抗体可阻断宿主细胞GTF2F1与病毒RNA聚合酶的结合，从而抑制疱疹病毒的转录复制，为抗病毒治疗提供了新思路。
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实际文献建议通过 **PubMed** 或 **Google Scholar** 以关键词“GTF2F1 antibody”检索，并筛选涉及抗体应用(如WB、IP、IHC等)的研究。
The GTF2F1 antibody targets the General Transcription Factor IIF subunit 1 (GTF2F1), a critical component of the RNA polymerase II transcription machinery. GTF2F1. also known as TFIIFα/RAP74. forms a heterodimer with GTF2F2 (TFIIFβ/RAP30) to create the transcription factor IIF (TFIIF) complex. This complex plays a central role in transcription initiation by stabilizing RNA polymerase II binding to promoter regions, facilitating promoter clearance, and regulating transcription elongation. GTF2F1 contains domains involved in interactions with RNA polymerase II and other transcription factors, such as TFIIB and TFIIH.
Antibodies against GTF2F1 are widely used in molecular biology research to study transcriptional regulation mechanisms. They enable detection and quantification of GTF2F1 in techniques like Western blotting, immunoprecipitation, and immunofluorescence. Additionally, these antibodies help investigate TFIIF's role in chromatin remodeling, DNA repair, and cell cycle progression. Dysregulation of GTF2F1 has been implicated in certain cancers and developmental disorders, making its antibody a tool for exploring disease-associated transcriptional abnormalities. Commercial GTF2F1 antibodies are typically raised in rabbits or mice using recombinant protein epitopes, with validation in specific experimental contexts required for optimal application.
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