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Rabbit Polyclonal RAD23B Antibody

  • 中文名: RAD23B抗体
  • 别    名: P58; HR23B; HHR23B
货号: IPDX02710
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/30-1/150 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

AliasesP58; HR23B; HHR23B
WB Predicted band size43 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Rat
ImmunogenFusion protein of human RAD23B
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是3篇关于RAD23B抗体的相关文献摘要(文献标题与内容为模拟生成,建议通过学术数据库核对原文):

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1. **文献名称**: *RAD23B interacts with XPC to promote nucleotide excision repair*

**作者**: Sugasawa, K. et al. (2001)

**摘要**: 研究证明RAD23B与XPC蛋白形成稳定复合物,通过抗体免疫共沉淀实验验证了两者在核苷酸切除修复(NER)中的功能协同性,揭示了RAD23B在DNA损伤识别中的支架作用。

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2. **文献名称**: *Ubiquitin receptors in DNA repair: RAD23B as a shuttle factor for proteasome targeting*

**作者**: Dantuma, N.P. et al. (2006)

**摘要**: 利用RAD23B特异性抗体,研究发现其通过UBL结构域介导受损DNA-蛋白复合物的泛素化标记,并协调蛋白酶体依赖性降解,强调了其在连接DNA修复与蛋白稳态中的双重功能。

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3. **文献名称**: *Structure-function analysis of RAD23B in transcription-coupled repair*

**作者**: Schulman, B.A. & Hoeijmakers, J.H. (2009)

**摘要**: 通过抗体阻断实验,揭示了RAD23B的XPC结合域和泛素结合域对维持转录偶联修复(TCR)的关键作用,并解析了其结构域突变导致的修复缺陷表型。

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4. **文献名称**: *RAD23B expression correlates with prostate cancer progression*

**作者**: Raman, M. et al. (2011)

**摘要**: 采用RAD23B抗体进行免疫组化分析,发现其在晚期前列腺癌组织中高表达,提示其可能作为DNA修复失调的潜在生物标志物,并与化疗耐药性相关。

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**注**: 以上文献信息为示例性质,实际引用时需通过PubMed或Web of Science等平台核实原文准确性。

背景信息

The RAD23B antibody is a crucial tool in studying the RAD23 homolog B protein, a key player in nucleotide excision repair (NER) and ubiquitin-proteasome pathways. RAD23B, along with its homolog RAD23A, functions as a shuttle factor by binding both damaged DNA via interactions with XPC (xeroderma pigmentosum complementation group C) and the 26S proteasome through its ubiquitin-like (UBL) domain. This dual role enables RAD23B to participate in DNA damage recognition and protein degradation. The protein also contains ubiquitin-associated (UBA) domains critical for binding polyubiquitinated substrates. RAD23B antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate its expression, localization, and interactions in DNA repair mechanisms, proteostasis, and cancer biology. Dysregulation of RAD23B has been implicated in xeroderma pigmentosum (XP), neurodegenerative disorders, and tumorigenesis, making its antibody valuable for diagnostic or functional studies. Researchers frequently validate RAD23B antibodies to ensure specificity, as RAD23A/B share structural homology. These antibodies help elucidate RAD23B's role in balancing DNA repair fidelity with protein quality control, particularly under genotoxic stress. Proper antibody selection depends on experimental goals, considering epitope regions and cross-reactivity with RAD23A.

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