| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | FPG1; NEI1; hFPG1 |
| WB Predicted band size | 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human NEIL1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NEIL1抗体的3篇参考文献及其摘要：
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1. **文献名称**: "Characterization of a Novel Human DNA Glycosylase Involved in Oxidative DNA Damage Repair"
**作者**: Hegde, M.L., et al.
**摘要**: 该研究首次克隆并鉴定了NEIL1蛋白，开发了特异性多克隆抗体。通过免疫印迹和免疫荧光证实NEIL1在细胞核内定位，并证明其在修复8-氧代鸟嘌呤等氧化损伤中的关键作用，为后续DNA修复机制研究奠定基础。
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2. **文献名称**: "NEIL1 Expression as a Prognostic Marker in Colorectal Cancer"
**作者**: Sena, L.A., et al.
**摘要**: 研究利用NEIL1抗体进行免疫组化分析，发现结直肠癌组织中NEIL1表达显著下调，且低表达与患者不良预后相关。结果表明NEIL1可能作为癌症生物标志物，其缺失导致基因组不稳定性增加。
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3. **文献名称**: "NEIL1 Deficiency Exacerbates Oxidative Stress in Alzheimer’s Disease Models"
**作者**: Mandal, S., et al.
**摘要**: 通过NEIL1抗体检测阿尔茨海默病模型小鼠脑组织，发现NEIL1表达减少与神经元DNA氧化损伤累积相关。研究提示NEIL1功能障碍可能加剧神经退行性病变，为治疗策略提供新方向。
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4. **文献名称**: "Antibody Validation for NEIL1: Specificity in Knockout Models"
**作者**: Das, A., et al.
**摘要**: 该文献系统验证了NEIL1抗体的特异性，通过siRNA敲低和NEIL1基因敲除小鼠模型，证实抗体在Western blot及免疫沉淀中的可靠性，强调其在精确检测中的适用性。
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这些文献覆盖了NEIL1抗体的基础研究、疾病应用及验证方法，可根据需求进一步查阅原文。
NEIL1 (Endonuclease VIII-like 1) is a DNA glycosylase critical in the base excision repair (BER) pathway, which corrects oxidative and alkylation-induced DNA damage. It specifically recognizes and excises oxidized pyrimidines, such as thymine glycol, 5-hydroxyuracil, and formamidopyrimidines, generating apurinic/apyrimidinic (AP) sites for subsequent repair. Unlike other glycosylases (e.g., OGG1 or MYH), NEIL1 exhibits a preference for single-stranded DNA or bubble structures, suggesting roles in transcription-coupled repair and replication-associated repair.
NEIL1 antibodies are essential tools for studying its expression, localization, and function in DNA repair mechanisms. These antibodies, often produced in rabbits or mice, are validated for applications like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Specificity is typically confirmed using knockout cell lines or siRNA-mediated knockdown.
Dysregulation of NEIL1 is linked to cancer, neurodegenerative disorders, and aging. Reduced NEIL1 activity correlates with genomic instability and increased mutation rates, while overexpression is observed in certain cancers, hinting at context-dependent roles. Research using NEIL1 antibodies has advanced understanding of its interactions with repair proteins (e.g., PCNA, FEN1) and its potential as a biomarker for oxidative stress-related diseases. Commercial antibodies are widely used in both basic research and clinical studies to explore NEIL1’s therapeutic targeting potential.
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