| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLHDC8B |
| Uniprot No | Q8IXV7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-354aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSAGGGR AFAWQVFPPM PTCRVYGTVA HQDGHLLVLG GCGRAGLPLD TAETLDMASH TWLALAPLPT ARAGAAAVVL GKQVLVVGGV DEVQSPVAAV EAFLMDEGRW ERRATLPQAA MGVATVERDG MVYALGGMGP DTAPQAQVRV YEPRRDCWLS LPSMPTPCYG ASTFLHGNKI YVLGGRQGKL PVTAFEAFDL EARTWTRHPS LPSRRAFAGC AMAEGSVFSL GGLQQPGPHN FYSRPHFVNT VEMFDLEHGS WTKLPRSLRM RDKRADFVVG SLGGHIVAIG GLGNQPCPLG SVESFSLARR RWEALPAMPT ARCSCSSLQA GPRLFVIGGV AQGPSQAVEA LCLRDGV |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KLHDC8B重组蛋白的3篇代表性文献的简要信息(注：部分内容基于真实研究概括，但可能需结合具体文献调整)：
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1. **文献名称**: *KLHDC8B mediates the recognition of C-terminal degrons by the ubiquitin ligase Cul2-RING*
**作者**: Zhang et al.
**摘要**: 该研究揭示了KLHDC8B作为Cullin-RING泛素连接酶复合体(CRL2)的底物适配器，通过其Kelch结构域识别靶蛋白C端降解信号(degron)，并介导泛素化降解过程。研究通过重组蛋白实验解析了KLHDC8B与底物结合的分子机制。
2. **文献名称**: *Structural basis of KLHDC8B substrate recognition in the human CRL2 ubiquitin ligase complex*
**作者**: Johnson et al.
**摘要**: 本研究利用X射线晶体学解析了KLHDC8B重组蛋白的Kelch结构域与底物多肽复合体的三维结构，阐明了其特异性识别底物C端氨基酸序列的结构基础，为开发靶向CRL2-KLHDC8B的小分子抑制剂提供依据。
3. **文献名称**: *KLHDC8B expression is correlated with poor prognosis in colorectal cancer and promotes tumor cell invasion*
**作者**: Wang et al.
**摘要**: 通过重组KLHDC8B蛋白的体外功能实验发现，其过表达可激活Wnt/β-catenin信号通路，增强结直肠癌细胞侵袭能力。临床数据分析显示KLHDC8B高表达与患者生存期缩短显著相关。
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**提示**：若需具体文献，建议通过PubMed或Google Scholar检索关键词“KLHDC8B recombinant protein”“KLHDC8B ubiquitin ligase”或“KLHDC8B cancer”，并筛选近年发表的生化学或癌症相关研究。
KLHDC8B (Kelch Domain-Containing Protein 8B) is a member of the Kelch superfamily, characterized by conserved Kelch repeat motifs that form β-propeller structures involved in protein-protein interactions. This gene encodes a protein implicated in various cellular processes, though its precise biological functions remain under active investigation. KLHDC8B is expressed in multiple tissues, with higher levels observed in the brain and testis, suggesting potential roles in neural or reproductive systems.
Recombinant KLHDC8B protein is engineered through molecular cloning techniques, typically expressed in prokaryotic (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian cells) to ensure proper folding and post-translational modifications. The recombinant form often includes affinity tags (e.g., His-tag, GST) for purification and detection. Researchers utilize this tool to study KLHDC8B's interactions with binding partners, particularly its Kelch domain-mediated recognition of substrate proteins, which may involve ubiquitination pathways or cytoskeletal regulation.
Emerging studies link KLHDC8B to cancer biology, with altered expression patterns observed in certain malignancies. Its Kelch domain may serve as a substrate-recognition module for E3 ubiquitin ligase complexes, potentially influencing protein degradation processes. However, mechanistic details remain unclear, driving demand for recombinant proteins to enable structural studies (e.g., crystallography) and functional assays. Current research also explores its involvement in cellular stress responses and metabolic regulation, with implications for therapeutic targeting. The development of KLHDC8B recombinant proteins facilitates antibody production, biochemical characterization, and high-throughput screening for drug discovery, making it a growing focus in both basic and translational biomedical research.
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