| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLF4 |
| Uniprot No | O43474-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-479aa |
| 氨基酸序列 | MRQPPGESDMAVSDALLPSFSTFASGPAGREKTLRQAGAPNNRWREELSH MKRLPPVLPGRPYDLAAATVATDLESGGAGAACGGSNLAPLPRRETEEFN DLLDLDFILSNSLTHPPESVAATVSSSASASSSSSPSSSGPASAPSTCSF TYPIRAGNDPGVAPGGTGGGLLYGRESAPPPTAPFNLADINDVSPSGGFV AELLRPELDPVYIPPQQPQPPGGGLMGKFVLKASLSAPGSEYGSPSVISV SKGSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLGAGPPLSNGHRP AAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYPS FLPDQMQPQVPPLHYQELMPPGSCMPEEPKPKRGRRSWPRKRTATHTCDY AGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKH TGHRPFQCQKCDRAFSRSDHLALHMKRHFESGGGGSPGRRRRRRRRRRR |
| 预测分子量 | 53 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors"**
- **作者**: Takahashi K, Yamanaka S.
- **摘要**: 研究证实KLF4与OCT4、SOX2、c-MYC组合可将体细胞重编程为多能干细胞，揭示了KLF4重组蛋白在细胞命运调控中的关键作用。
2. **"KLF4 Suppresses Tumorigenesis in Gastrointestinal Cancers through Inhibition of Cell Proliferation"**
- **作者**: Wei D, Kanai M, Jia Z.
- **摘要**: 探讨KLF4重组蛋白通过调控细胞周期蛋白(如p21)抑制胃癌细胞增殖，强调其作为肿瘤抑制因子的潜在治疗价值。
3. **"Purification and Functional Analysis of Recombinant KLF4 Protein for Direct Cellular Reprogramming"**
- **作者**: Kim J, Ormanaly K, Lee JH.
- **摘要**: 描述KLF4重组蛋白的高效纯化方法，并验证其与DNA结合及激活多能性基因的能力，为再生医学提供技术基础。
4. **"Dual Role of KLF4 in Oxidative Stress-Induced Cellular Senescence"**
- **作者**: Rowland BD, Peeper DS.
- **摘要**: 揭示KLF4重组蛋白在氧化应激中既促进衰老相关基因表达，又参与DNA修复的双重功能，机制复杂性与细胞类型相关。
(注：上述文献信息为示例性概括，实际引用需核对真实出版物。)
KLF4 (Krüppel-like factor 4) is a zinc finger transcription factor pivotal in regulating cellular processes such as pluripotency, differentiation, proliferation, and apoptosis. Initially identified for its role in gastrointestinal epithelial cell differentiation, KLF4 gained prominence as one of the Yamanaka factors (alongside Oct4. Sox2. and c-Myc) capable of reprogramming somatic cells into induced pluripotent stem cells (iPSCs). This breakthrough highlighted its dual functionality, acting as both a tumor suppressor and oncogene depending on cellular context.
Recombinant KLF4 protein is engineered using expression systems like *E. coli* or mammalian cells, followed by purification to ensure bioactivity. Its structure includes three conserved Cys2/His2 zinc fingers that bind GC-rich DNA sequences, enabling regulation of target genes like p21 and Nanog. Researchers leverage recombinant KLF4 to study cellular reprogramming mechanisms, tissue regeneration, and disease modeling. In cancer biology, it helps dissect context-dependent roles—suppressing tumor growth in certain cancers (e.g., colorectal) while promoting progression in others (e.g., breast).
Despite its therapeutic potential, challenges remain in optimizing delivery efficiency and minimizing off-target effects. Current applications extend to organoid development, drug screening, and understanding epigenetic regulation. As a tool, recombinant KLF4 continues to bridge basic research and translational medicine, offering insights into stem cell biology and precision therapies.
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