| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLF12 |
| Uniprot No | Q9Y4X4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-402aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMNIHMKRKTIKNINTFENRMLMLDGMP AVRVKTELLESEQGSPNVHNYPDMEAVPLLLNNVKGEPPEDSLSVDHFQT QTEPVDLSINKARTSPTAVSSSPVSMTASASSPSSTSTSSSSSSRLASSP TVITSVSSASSSSTVLTPGPLVASASGVGGQQFLHIIHPVPPSSPMNLQS NKLSHVHRIPVVVQSVPVVYTAVRSPGNVNNTIVVPLLEDGRGHGKAQMD PRGLSPRQSKSDSDDDDLPNVTLDSVNETGSTALSIARAVQEVHPSPVSR VRGNRMNNQKFPCSISPFSIESTRRQRRSESPDSRKRRIHRCDFEGCNKV YTKSSHLKAHRRTHTGEKPYKCTWEGCTWKFARSDELTRHYRKHTGVKPF KCADCDRSFSRSDHLALHRRRHMLV |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KLF12重组蛋白的3篇参考文献及其摘要内容：
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1. **文献名称**: *"KLF12 Regulates Human Endometrial Stromal Cell Decidualization via Reprogramming Transcriptome Remodeling"*
**作者**: Li et al.
**摘要**: 该研究利用重组KLF12蛋白分析其在子宫内膜基质细胞蜕膜化中的作用，发现KLF12通过直接结合靶基因启动子区域，抑制与细胞增殖相关的基因(如Cyclin D1)，并激活蜕膜化标志基因(PRL和IGFBP1)，揭示了其在转录重编程中的关键调控机制。
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2. **文献名称**: *"Structural and Functional Analysis of KLF12 DNA-Binding Domain"*
**作者**: Garcia-Gutierrez et al.
**摘要**: 通过重组表达纯化KLF12的锌指DNA结合域，结合X射线晶体学解析其三维结构，发现其特异性识别DNA基序的分子机制。实验进一步验证KLF12通过与靶基因启动子中的CACCC盒结合，调控胚胎发育相关通路。
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3. **文献名称**: *"KLF12 Suppresses Breast Cancer Metastasis by Antagonizing EMT Transcription Factors"*
**作者**: Wang et al.
**摘要**: 研究利用重组KLF12蛋白进行体外功能实验，证明其通过竞争性抑制EMT(上皮-间质转化)关键转录因子(如ZEB1和SNAI1)的DNA结合能力，抑制乳腺癌细胞迁移和侵袭，为KLF12作为转移抑制因子提供了分子证据。
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这些研究均围绕KLF12重组蛋白的功能展开，涵盖转录调控、结构解析及疾病机制，可作为相关领域研究的基础参考。
Krüppel-like factor 12 (KLF12) is a member of the Krüppel-like factor (KLF) family of transcription factors, characterized by conserved C-terminal zinc finger domains that mediate DNA binding. KLF12 primarily functions as a transcriptional repressor, regulating genes involved in cell proliferation, differentiation, and apoptosis. It binds to GC-rich promoter regions, often interacting with co-repressors like CtBP to suppress target gene expression. KLF12 plays roles in diverse biological processes, including embryonic development, tissue homeostasis, and cancer progression. Studies link its dysregulation to diseases such as cancers (e.g., gastric, breast) and cardiovascular disorders.
Recombinant KLF12 protein is engineered using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional protein for research. Its production enables mechanistic studies on DNA-protein interactions, transcriptional regulation, and signaling pathways. Researchers use it to explore KLF12’s role in epithelial-mesenchymal transition (EMT), metastasis, and drug resistance. Notably, KLF12 interacts with proteins like AP-2α, modulating shared targets in developmental and pathological contexts. Structural studies of recombinant KLF12 help map functional domains and mutations affecting its activity. As a potential therapeutic target, understanding KLF12’s molecular behavior via recombinant tools may inform strategies to modulate its activity in disease states. Current challenges include elucidating context-dependent regulatory networks and post-translational modifications influencing its function.
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