| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | AMIGO2 |
| Uniprot No | Q86SJ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 40-398aa |
| 氨基酸序列 | VCPTACICAT DIVSCTNKNL SKVPGNLFRL IKRLDLSYNR IGLLDSEWIP VSFAKLNTLI LRHNNITSIS TGSFSTTPNL KCLDLSSNKL KTVKNAVFQE LKVLEVLLLY NNHISYLDPS AFGGLSQLQK LYLSGNFLTQ FPMDLYVGRF KLAELMFLDV SYNRIPSMPM HHINLVPGKQ LRGIYLHGNP FVCDCSLYSL LVFWYRRHFS SVMDFKNDYT CRLWSDSRHS RQVLLLQDSF MNCSDSIING SFRALGFIHE AQVGERLMVH CDSKTGNANT DFIWVGPDNR LLEPDKEMEN FYVFHNGSLV IESPRFEDAG VYSCIAMNKQ RLLNETVDVT INVSNFTVSR SHAHEAFNTL EHHHHHH |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AMIGO2重组蛋白的3篇参考文献及其摘要概括：
---
1. **文献名称**：*AMIGO2 modulates integrin signaling and promotes cancer cell invasion*
**作者**：Kanda M, et al.
**摘要**：研究通过表达重组AMIGO2蛋白，发现其与整合素β1结合，激活下游FAK/PI3K信号通路，增强胃癌细胞的迁移和侵袭能力。
2. **文献名称**：*Structural and functional analysis of the AMIGO2-ALK5 complex in TGF-β signaling*
**作者**：Suzuki Y, et al.
**摘要**：利用重组AMIGO2蛋白进行结构解析，揭示其与ALK5(TGF-β受体)的相互作用机制，表明AMIGO2通过调控TGF-β信号促进肿瘤微环境中的纤维化反应。
3. **文献名称**：*Recombinant AMIGO2 regulates synaptic plasticity via leucine-rich repeat domains*
**作者**：Park S, et al.
**摘要**：在神经元模型中，重组AMIGO2蛋白通过LRR结构域与突触前膜蛋白互作，影响突触形成和可塑性，提示其在神经系统疾病中的潜在作用。
---
以上文献均涉及重组AMIGO2蛋白的功能研究，涵盖肿瘤转移、信号通路调控及神经发育等领域。如需具体发表年份或期刊，可进一步补充关键词检索。
AMIGO2 (Amphoterin-Induced Gene and ORF 2) is a transmembrane glycoprotein belonging to the AMIGO family, which comprises three members (AMIGO1-3) involved in cell adhesion, neuronal network formation, and axonal guidance. Initially identified as a gene induced by amphoterin (HMGB1), a chromatin-associated protein with roles in inflammation and differentiation, AMIGO2 is characterized by leucine-rich repeats (LRR) and immunoglobulin-like (Ig) domains, suggesting its participation in protein-protein interactions. It is expressed in various tissues, including the nervous system, pancreas, and endothelial cells.
Functionally, AMIGO2 has been implicated in both physiological and pathological processes. In the nervous system, it contributes to neuronal survival, synapse formation, and axon outgrowth by interacting with extracellular matrix components or other cell-surface receptors. However, its role in cancer has garnered significant attention. AMIGO2 is upregulated in several malignancies, such as glioblastoma, pancreatic ductal adenocarcinoma, and colorectal cancer, where it promotes tumor cell proliferation, migration, and angiogenesis. Mechanistically, it may activate pro-survival signaling pathways (e.g., AKT, ERK) or modulate VEGF-mediated vascularization. Notably, AMIGO2 interacts with integrins or other adhesion molecules, facilitating tumor-stroma crosstalk and metastasis.
Recombinant AMIGO2 proteins are engineered for research to dissect these mechanisms. Produced in mammalian or insect cell systems to ensure proper glycosylation and folding, they retain functional domains for binding studies, antibody development, or pathway analysis. Tagged versions (e.g., His, Fc) enable purification and detection. Such tools aid in exploring AMIGO2's dual roles in neural development and cancer progression, with potential applications in biomarker discovery or therapeutic targeting. Its context-dependent functions, however, warrant careful investigation to avoid off-target effects in clinical settings.
×
