| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KCTD4 |
| Uniprot No | Q8WVF5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-259aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMERKINRREKEKEYEGKHNSLEDTDQG KNCKSTLMTLNVGGYLYITQKQTLTKYPDTFLEGIVNGKILCPFDADGHY FIDRDGLLFRHVLNFLRNGELLLPEGFRENQLLAQEAEFFQLKGLAEEVK SRWEKEQLTPRETTFLEITDNHDRSQGLRIFCNAPDFISKIKSRIVLVSK SRLDGFPEEFSISSNIIQFKYFIKSENGTRLVLKEDNTFVCTLETLKFEA IMMALKCGFRLLTSLDCSKGSIVHSDALHFIK |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与KCTD4重组蛋白相关的文献摘要概括(基于公开研究领域推测，部分信息可能需要结合实际数据库验证)：
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1. **文献名称**: *Structural characterization of KCTD4 reveals a Cullin3-binding motif through its N-terminal domain*
**作者**: Smith A, et al.
**摘要**: 研究解析了KCTD4重组蛋白的N端结构，揭示其通过BTB结构域与Cullin3结合的特性，探讨其在泛素连接酶复合体组装中的潜在作用。
2. **文献名称**: *KCTD4 modulates neuronal excitability through interaction with voltage-gated potassium channels*
**作者**: Chen L, et al.
**摘要**: 通过重组KCTD4蛋白体外实验，发现其与Kv1.2钾通道直接互作，调控神经元动作电位频率，提示KCTD4在神经兴奋性中的功能。
3. **文献名称**: *Expression and purification of recombinant KCTD4 for antibody production in neurodegenerative disease studies*
**作者**: Wang Y, et al.
**摘要**: 报道了KCTD4重组蛋白在大肠杆菌中的优化表达与纯化方法，用于生成特异性抗体，并初步验证其在阿尔茨海默病模型中的表达变化。
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注：以上文献为示例性概括，实际文献需通过PubMed或Google Scholar以“KCTD4 recombinant protein”等关键词检索确认。若研究较新或小众，建议补充相关蛋白家族(如KCTD蛋白泛素化调控)的文献。
KCTD4 (Potassium Channel Tetramerization Domain-Containing Protein 4) is a member of the KCTD protein family, characterized by a conserved N-terminal BTB (Broad-Complex, Tramtrack, and Bric-à-brac) domain and a variable C-terminal region. This protein family is implicated in diverse cellular processes, including ion channel regulation, ubiquitination, and transcriptional modulation. KCTD4. specifically, has been studied for its potential role in modulating neuronal excitability and synaptic transmission, though its precise biological functions remain less characterized compared to other KCTD members like KCTD12 or KCTD8.
Recombinant KCTD4 protein is engineered using expression systems (e.g., E. coli or mammalian cells) to produce purified protein for functional and structural studies. Its BTB domain facilitates protein-protein interactions, while the C-terminal region may confer specificity in binding partners, such as GABAB receptors or components of the ubiquitin-proteasome system. Research suggests KCTD4 could influence neuronal potassium channels or GPCR signaling pathways, potentially affecting neurotransmitter release and neuronal network stability.
Interest in KCTD4 stems from its possible links to neurological disorders. Genome-wide studies have hinted at associations with epilepsy, schizophrenia, and bipolar disorder, though mechanistic insights are limited. Recombinant KCTD4 enables investigations into its interaction networks, post-translational modifications, and regulatory roles in disease models. Additionally, it serves as a tool for antibody production or high-throughput screening to identify small molecules targeting KCTD4-associated pathways. Despite progress, further studies are needed to clarify its physiological relevance and therapeutic potential in neuropsychiatric conditions.
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