| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | AMPD2 |
| Uniprot No | Q01433 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 236-879aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSDLLDAAKSVVRALFIREKYMALSLQSF CPTTRRYLQQLAEKPLETRTYEQGPDTPVSADAPVHPPALEQHPYEHCEP STMPGDLGLGLRMVRGVVHVYTRREPDEHCSEVELPYPDLQEFVADVNVL MALIINGPIKSFCYRRLQYLSSKFQMHVLLNEMKELAAQKKVPHRDFYNI RKVDTHIHASSCMNQKHLLRFIKRAMKRHLEEIVHVEQGREQTLREVFES MNLTAYDLSVDTLDVHADRNTFHRFDKFNAKYNPIGESVLREIFIKTDNR VSGKYFAHIIKEVMSDLEESKYQNAELRLSIYGRSRDEWDKLARWAVMHR VHSPNVRWLVQVPRLFDVYRTKGQLANFQEMLENIFLPLFEATVHPASHP ELHLFLEHVDGFDSVDDESKPENHVFNLESPLPEAWVEEDNPPYAYYLYY TFANMAMLNHLRRQRGFHTFVLRPHCGEAGPIHHLVSAFMLAENISHGLL LRKAPVLQYLYYLAQIGIAMSPLSNNSLFLSYHRNPLPEYLSRGLMVSLS TDDPLQFHFTKEPLMEEYSIATQVWKLSSCDMCELARNSVLMSGFSHKVK SHWLGPNYTKEGPEGNDIRRTNVPDIRVGYRYETLCQELALITQAVQSEM LETIPEEAGITMSPGPQ |
| 预测分子量 | 77 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于AMPD2重组蛋白研究的参考文献概览(注：文献标题与作者为模拟示例，实际检索需通过学术数据库验证)：
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1. **标题**：*Expression and Purification of Recombinant Human AMPD2 in E. coli*
**作者**：Zhang L et al.
**摘要**：研究报道了人源AMPD2基因在大肠杆菌中的重组表达及镍柱亲和纯化策略，获得可溶性蛋白并验证其腺苷单磷酸脱氨酶活性，为后续酶动力学研究提供材料。
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2. **标题**：*Structural Insights into AMPD2 Function via Recombinant Protein Crystallography*
**作者**：Itoh K et al.
**摘要**：通过昆虫细胞表达系统获得高纯度AMPD2重组蛋白，利用X射线晶体学解析其三维结构，揭示了底物结合口袋的关键氨基酸残基，阐明了其催化机制。
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3. **标题**：*AMPD2 Deficiency Model: Recombinant Protein Rescue in Zebrafish*
**作者**：Wang Y et al.
**摘要**：构建斑马鱼AMPD2敲除模型后，体外表达重组AMPD2并通过显微注射回补，证实该蛋白可挽救模型动物的心脏发育缺陷，提示其在嘌呤代谢中的生理重要性。
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**建议**：实际文献需通过PubMed/Google Scholar以“AMPD2 recombinant protein”或“AMPD2 purification”等关键词检索，并筛选实验类论文。如需具体文献协助，可提供数据库访问权限进一步查询。
**Background of AMPD2 Recombinant Protein**
Adenosine monophosphate deaminase 2 (AMPD2) is a critical enzyme in purine metabolism, catalyzing the irreversible conversion of adenosine monophosphate (AMP) to inosine monophosphate (IMP) while releasing ammonia. This reaction plays a vital role in maintaining cellular energy homeostasis, particularly in tissues with high energy demands, such as skeletal muscle, brain, and heart. AMPD2. encoded by the *AMPD2* gene, is one of three isoforms in humans (AMPD1. AMPD2. AMPD3), each exhibiting tissue-specific expression patterns. Unlike AMPD1 (primarily muscular) and AMPD3 (erythroid-specific), AMPD2 is ubiquitously expressed, suggesting broader regulatory roles in cellular metabolism and signaling.
Dysregulation of AMPD2 has been implicated in various pathological conditions. Mutations in *AMPD2* are linked to hereditary spastic paraplegia (HSP), a neurodegenerative disorder characterized by progressive lower-limb spasticity and weakness. Studies suggest that AMPD2 deficiency disrupts purine recycling, leading to impaired neuronal function and axonal degeneration. Additionally, altered AMPD2 activity may influence cancer progression, as purine metabolism is crucial for proliferating cells.
Recombinant AMPD2 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables detailed biochemical and structural studies. Its production typically involves cloning the *AMPD2* cDNA into expression vectors, followed by purification using affinity chromatography. Recombinant AMPD2 is instrumental in elucidating enzyme kinetics, substrate specificity, and interactions with regulatory molecules. It also serves as a tool for drug discovery, particularly in targeting metabolic disorders or neurodegenerative diseases.
Overall, AMPD2 recombinant protein bridges gaps in understanding AMPD2’s physiological roles and therapeutic potential, offering insights into metabolic regulation and disease mechanisms.
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