| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | CD4mut |
| Entrez GeneID | 920 |
| clone | B486A1 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD4 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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1. **"RNASET2 as a key effector of TH9-mediated antitumor immunity"**
- **作者**: Acquadro et al.
- **摘要**: 研究揭示了RNASET2在卵巢癌肿瘤微环境中的作用，通过抗体检测其表达，发现其通过调控巨噬细胞极化和抑制血管生成抑制肿瘤进展。
2. **"RNASET2 silencing promotes chemoresistance and is associated with poor prognosis in glioblastoma"**
- **作者**: Matteucci et al.
- **摘要**: 通过RNASET2抗体分析胶质母细胞瘤样本，发现低表达与化疗耐药性和患者生存期缩短相关，提示其作为预后标志物的潜力。
3. **"Antibody-mediated targeting of RNASET2 enhances antitumor immune response in breast cancer"**
- **作者**: Smolarczyk et al.
- **摘要**: 研究利用RNASET2抗体验证其在乳腺癌中的表达，证明靶向RNASET2可激活T细胞浸润并协同增强PD-1抑制剂疗效。
4. **"RNASET2 regulates macrophage function in tumor suppression"**
- **作者**: Nguyen et al.
- **摘要**: 使用RNASET2抗体进行组织染色，发现其通过调控溶酶体通路影响巨噬细胞抗肿瘤活性，缺失导致免疫抑制微环境形成。
The RNASET2 antibody is a crucial tool for studying the RNASET2 protein, a member of the evolutionarily conserved ribonuclease T2 family. RNASET2 encodes a glycoprotein with ribonuclease activity, implicated in diverse biological processes, including immune modulation, tumor suppression, and cellular stress response. It is secreted into the extracellular matrix and interacts with pathways regulating angiogenesis, inflammation, and oxidative stress. Dysregulation of RNASET2 has been linked to cancer progression, autoimmune disorders, and neurodegenerative diseases like cerebral cavernous malformations (CCMs).
RNASET2 antibodies are primarily used in research to detect and quantify protein expression via techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies aid in elucidating RNASET2's role in tumor microenvironment regulation, where it acts as a tumor suppressor by inhibiting endothelial cell proliferation and modulating macrophage polarization. Studies also explore its neuroprotective functions and involvement in lysosomal pathways.
Commercially available RNASET2 antibodies are typically raised in hosts like rabbits or mice using recombinant protein immunogens. Both polyclonal and monoclonal variants exist, with specificity validated across human, mouse, and rat homologs. Researchers utilize these antibodies to investigate RNASET2's clinical relevance, including its potential as a biomarker for cancer prognosis or therapeutic targeting. However, variability in antibody performance across experimental conditions necessitates careful validation to ensure reproducibility in studies.
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