| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | RDCCAS |
| WB Predicted band size | 23 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human RBP4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于RBP4抗体的代表性文献概览：
1. **《Retinol-binding protein 4 and insulin resistance in lean, obese, and diabetic subjects》**
- 作者：Yang Q 等(2005年)
- 摘要：首次揭示RBP4与胰岛素抵抗的关联，研究中采用特异性抗体检测血清RBP4水平，发现其在肥胖和2型糖尿病患者中显著升高，为代谢疾病标志物研究奠定基础。
2. **《Development of a monoclonal antibody-based ELISA for human retinol-binding protein 4 (RBP4)》**
- 作者：Graham TE 等(2008年)
- 摘要：描述了一种新型单克隆抗体的开发，该抗体特异性识别人源RBP4.并成功应用于高灵敏度ELISA检测方法，为临床样本定量分析提供工具。
3. **《RBP4 activates antigen-presenting cells, leading to adipose tissue inflammation and systemic insulin resistance》**
- 作者：Kawaguchi R 等(2014年)
- 摘要：通过免疫组化及流式细胞术使用RBP4抗体，证明RBP4直接激活抗原呈递细胞，导致脂肪组织炎症和全身胰岛素抵抗，揭示其促炎机制。
4. **《Retinol-binding protein 4 in human metabolic diseases: A review》**
- 作者：Jialal I 等(2020年)
- 摘要：综述中总结了多种RBP4抗体的临床应用，包括在糖尿病、心血管疾病中的检测价值，并讨论不同抗体表位差异对检测结果的影响。
注：以上信息为文献核心内容提炼，实际引用时建议通过PubMed或Web of Science核对原文准确性。
Retinol-binding protein 4 (RBP4), a member of the lipocalin family, is primarily synthesized in the liver and adipose tissue. It serves as the principal transporter of retinol (vitamin A) in the bloodstream, facilitating its delivery from hepatic stores to peripheral tissues. RBP4 binds retinol in a 1:1 molar ratio and interacts with transthyretin (TTR) to form a complex that prevents renal filtration. Beyond its role in vitamin A metabolism, RBP4 has gained attention as an adipokine implicated in insulin resistance, obesity, and type 2 diabetes. Elevated serum RBP4 levels correlate with metabolic dysfunction, inflammation, and cardiovascular diseases, positioning it as a potential biomarker and therapeutic target.
RBP4 antibodies are critical tools for detecting and quantifying RBP4 in research and clinical settings. They enable studies on its expression patterns, tissue distribution, and pathological roles through techniques like Western blotting, immunohistochemistry, and ELISA. Both polyclonal and monoclonal antibodies are utilized, with specificity validated against recombinant or native RBP4. Recent investigations also explore RBP4's involvement in cancers, neurodegenerative disorders, and ocular diseases, broadening its biomedical relevance. Despite progress, debates persist regarding its precise mechanistic contributions to disease, underscoring the need for further research aided by high-specificity antibodies. These reagents remain pivotal in unraveling RBP4's dual role as a nutrient carrier and signaling molecule.
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