| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | MMTN; B17.2L; mimitin; NDUFA12L |
| WB Predicted band size | 20 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human NDUFAF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NDUFAF2抗体的3篇参考文献示例(注：以下为模拟内容，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *NDUFAF2 functions as a mitochondrial complex I assembly factor and its deficiency causes Leigh syndrome*
**作者**: Ogilvie, I., et al.
**摘要**: 本研究通过Western blot和免疫荧光技术，利用NDUFAF2特异性抗体，揭示了其在复合物I组装中的关键作用。研究发现NDUFAF2突变导致线粒体功能异常，与早发性Leigh综合征相关。
2. **文献名称**: *Development of a monoclonal antibody against human NDUFAF2 for mitochondrial disease diagnostics*
**作者**: Chen, L., et al.
**摘要**: 报道了一种新型NDUFAF2单克隆抗体的开发，并通过免疫印迹和免疫组化验证其特异性。该抗体可用于线粒体病患者样本中NDUFAF2蛋白水平的检测，辅助临床诊断。
3. **文献名称**: *Tissue-specific expression profiling of NDUFAF2 in mammalian organs using immunohistochemistry*
**作者**: Sato, M., et al.
**摘要**: 利用NDUFAF2多克隆抗体对小鼠多种组织进行免疫组化分析，发现其在高能量需求器官(如心脏、肝脏)中高表达，提示其在维持线粒体能量代谢中的组织特异性功能。
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如需获取真实文献，建议在PubMed或Google Scholar中搜索关键词“NDUFAF2 antibody”、“NDUFAF2 immunohistochemistry”或结合疾病名称(如“mitochondrial complex I deficiency”)进行检索。
NDUFAF2 (NADH:ubiquinone oxidoreductase complex assembly factor 2) is a mitochondrial protein critical for the assembly and stability of mitochondrial complex I, a key component of the electron transport chain (ETC) responsible for ATP production. This protein, also known as human complex I intermediate-associated protein 30 (hCIA30), facilitates the proper maturation of complex I subunits and coordinates their integration into the functional holoenzyme. Dysregulation of NDUFAF2 has been linked to mitochondrial disorders, particularly Leigh syndrome, a severe neurodegenerative condition characterized by impaired energy metabolism.
Antibodies targeting NDUFAF2 are essential tools for studying complex I biogenesis, mitochondrial dysfunction, and related pathologies. They enable detection of NDUFAF2 expression levels via Western blotting, immunofluorescence, or immunohistochemistry, aiding in the diagnosis of mitochondrial diseases and research into their molecular mechanisms. Additionally, these antibodies help investigate the protein's role in cellular processes like apoptosis, oxidative stress response, and metabolic adaptation. Recent studies also explore NDUFAF2's potential involvement in cancer progression and neurodegenerative diseases, as altered ETC function is increasingly recognized in these contexts. Validated antibodies with high specificity are crucial for distinguishing NDUFAF2 from homologous proteins and ensuring accurate experimental outcomes in both clinical and research settings.
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