| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | PAB1; PABP; PABP1; PABPC2; PABPL1 |
| WB Predicted band size | 71 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PABPC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及PABPC1抗体应用的参考文献摘要：
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1. **"PABPC1 interacts with the ORF7 of porcine reproductive and respiratory syndrome virus and mediates viral replication"**
*Author: Li et al.*
研究利用PABPC1抗体通过免疫共沉淀(Co-IP)和Western blot验证PABPC1与猪繁殖与呼吸综合征病毒(PRRSV)ORF7蛋白的相互作用，揭示其通过调控病毒复制影响宿主感染机制。
2. **"Stress granule assembly is regulated by poly(ADP-ribose)-dependent protein phase separation"**
*Author: Hofmann et al.*
通过免疫荧光和Western blot使用PABPC1抗体标记应激颗粒(stress granules)，发现PARP1依赖的蛋白相变调控应激颗粒形成，PABPC1作为应激颗粒标志物用于动态观察。
3. **"Cytoplasmic poly(A)-binding protein deficiency alters synaptic plasticity and translation via mTOR signaling"**
*Author: Gkogkas et al.*
在小鼠模型中，采用PABPC1抗体进行脑组织免疫组化及蛋白分析，发现PABPC1缺失通过mTOR通路影响突触可塑性和翻译调控，与神经退行性疾病相关。
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这些文献均通过PABPC1抗体开展实验(如Western blot、免疫共沉淀、免疫荧光等)，研究其在病毒感染、细胞应激及神经调控中的功能。如需具体期刊信息或发表年份，可进一步补充检索。
The poly(A)-binding protein cytoplasmic 1 (PABPC1) is a highly conserved RNA-binding protein critical for post-transcriptional gene regulation. It binds the poly(A) tails of eukaryotic mRNAs, facilitating mRNA stability, translation initiation, and deadenylation. PABPC1 interacts with translation machinery components like eIF4G and the cytoplasmic deadenylase complex, playing a central role in mRNA circularization and translation efficiency. Its expression is ubiquitous across tissues, with elevated levels in cells requiring high protein synthesis, such as neurons and rapidly proliferating cells.
Antibodies targeting PABPC1 are essential tools for studying mRNA metabolism, translational control, and stress granule dynamics. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess PABPC1 localization, expression changes, or protein-RNA interactions. Dysregulation of PABPC1 has been implicated in cancers, viral infections (e.g., enteroviruses targeting its function), and neurodegenerative diseases like amyotrophic lateral sclerosis (ALS). Research using PABPC1 antibodies has also uncovered its role in cellular stress responses, such as forming cytoplasmic aggregates during oxidative stress or viral-induced translation shutdown. These antibodies are vital for exploring PABPC1’s molecular interactions and validating knockdown/knockout models in disease mechanisms.
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